Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   L6432_RS08090 Genome accession   NZ_AP025339
Coordinates   1623494..1624123 (-) Length   209 a.a.
NCBI ID   WP_025811128.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain J25TS1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618494..1629123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6432_RS08070 holA 1619053..1620078 (-) 1026 WP_023855252.1 DNA polymerase III subunit delta -
  L6432_RS08075 - 1620308..1620439 (+) 132 WP_003183686.1 YqzM family protein -
  L6432_RS08080 - 1620464..1622851 (-) 2388 WP_025811129.1 DNA internalization-related competence protein ComEC/Rec2 -
  L6432_RS08085 - 1622856..1623425 (-) 570 WP_020452273.1 ComE operon protein 2 -
  L6432_RS08090 comEA 1623494..1624123 (-) 630 WP_025811128.1 helix-hairpin-helix domain-containing protein Machinery gene
  L6432_RS08095 comER 1624206..1625027 (+) 822 WP_043054399.1 late competence protein ComER -
  L6432_RS08100 - 1625098..1625853 (-) 756 WP_023855255.1 class I SAM-dependent DNA methyltransferase -
  L6432_RS08105 rsfS 1625850..1626206 (-) 357 WP_023855256.1 ribosome silencing factor -
  L6432_RS08110 yqeK 1626220..1626783 (-) 564 WP_025811126.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  L6432_RS08115 - 1626773..1627342 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  L6432_RS08120 yhbY 1627361..1627651 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  L6432_RS08125 aroE 1627645..1628481 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22569.46 Da        Isoelectric Point: 4.9006

>NTDB_id=91320 L6432_RS08090 WP_025811128.1 1623494..1624123(-) (comEA) [Bacillus paralicheniformis strain J25TS1]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKRKKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSAELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=91320 L6432_RS08090 WP_025811128.1 1623494..1624123(-) (comEA) [Bacillus paralicheniformis strain J25TS1]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGAAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
GATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGCGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTTCCATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(145-207)

SLBB domain (SLBB)

(69-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526
  comEA Staphylococcus aureus MW2

36.818

100

0.388
  comEA Staphylococcus aureus N315

36.818

100

0.388

Multiple sequence alignment