Detailed information NTG0912423

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: U0037_RS03995
• Genome accession: NZ_CP140009
• Coordinates: 890023..890889 (-)
• Length: 288 a.a.
• NCBI ID: WP_012722159.1
• Uniprot ID: -
• Organism: Pseudomonas marginalis strain SBW25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 885023..895889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0037_RS03960 (U0037_03960)	-	885908..887206 (-)	1299	WP_012722153.1	NAD(P)/FAD-dependent oxidoreductase	-
U0037_RS03965 (U0037_03965)	-	887267..887440 (-)	174	WP_012722154.1	DUF3094 family protein	-
U0037_RS03970 (U0037_03970)	-	887495..888124 (-)	630	WP_010213242.1	DUF1780 domain-containing protein	-
U0037_RS03975 (U0037_03975)	-	888229..888918 (+)	690	WP_012722155.1	energy-coupling factor ABC transporter permease	-
U0037_RS03980 (U0037_03980)	-	888994..889209 (+)	216	WP_012722156.1	hypothetical protein	-
U0037_RS03985 (U0037_03985)	yacG	889206..889406 (-)	201	WP_012722157.1	DNA gyrase inhibitor YacG	-
U0037_RS03990 (U0037_03990)	coaE	889403..890026 (-)	624	WP_012722158.1	dephospho-CoA kinase	-
U0037_RS03995 (U0037_03995)	pilD	890023..890889 (-)	867	WP_012722159.1	prepilin peptidase	Machinery gene
U0037_RS04000 (U0037_04000)	pilC	890886..892094 (-)	1209	WP_043204767.1	type II secretion system F family protein	Machinery gene
U0037_RS04005 (U0037_04005)	-	892297..892710 (+)	414	WP_043206123.1	pilin	-
U0037_RS04010 (U0037_04010)	-	892747..894147 (+)	1401	WP_012722162.1	O-antigen ligase family protein	-
U0037_RS04020 (U0037_04020)	-	894428..894733 (+)	306	WP_012722163.1	DUF6388 family protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31792.94 Da
• Isoelectric Point: 7.9972

>NTDB_id=912423 U0037_RS03995 WP_012722159.1 890023..890889(-) (pilD) [Pseudomonas marginalis strain SBW25]
MNLLLVEQPWAFVAMAGVLGLIVGSFLNVLVWRLPKMLEREWRAQAHEVLGLPAEPAGPTYNLMHPNSCCPHCNHPIRPW
ENIPLLSYLFLRGRCAHCQGPISARYPLTELACGLISAWVAWHFGFGWQAGAMLVLSWGLLAMSLIDADHQLLPDALVMP
LLWLGLIVNALGLLTTLPDALWGAVIGYMSLWSVFWLFKLATGKDGMGHGDFKLLALLGAWGGWQILPMTLLISSLVGVF
VGLILMRLRKARASTPMPFGPYLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=912423 U0037_RS03995 WP_012722159.1 890023..890889(-) (pilD) [Pseudomonas marginalis strain SBW25]
TTGAACCTGCTATTGGTTGAGCAGCCCTGGGCTTTTGTGGCGATGGCCGGGGTGCTGGGGCTGATCGTCGGCAGTTTTCT
CAACGTGCTGGTGTGGCGCCTGCCGAAAATGCTCGAACGGGAATGGCGCGCCCAGGCCCACGAAGTGCTCGGCTTGCCCG
CTGAGCCTGCCGGGCCGACCTACAACCTGATGCACCCCAACTCCTGCTGCCCGCACTGCAACCATCCGATACGCCCATGG
GAAAATATCCCGTTGCTCAGTTACCTGTTTCTGCGCGGCCGCTGCGCCCATTGCCAAGGCCCGATCAGCGCCCGCTATCC
ACTCACTGAACTGGCGTGCGGGTTGATCTCGGCCTGGGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGGCGCGATGC
TGGTGCTGAGTTGGGGCTTGTTGGCGATGAGCCTGATCGATGCCGACCATCAACTGTTGCCGGATGCGCTGGTGATGCCG
CTGCTCTGGCTGGGACTGATCGTCAACGCGCTTGGCCTGCTGACGACGCTACCCGATGCCCTCTGGGGCGCGGTTATCGG
GTACATGAGCCTGTGGAGCGTATTCTGGCTGTTCAAGCTGGCCACCGGCAAGGACGGCATGGGCCACGGCGACTTCAAGC
TGCTGGCACTGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGATGACACTGCTGATATCGTCGCTGGTCGGGGTGTTC
GTGGGCTTGATCCTGATGCGCCTGCGCAAGGCCCGGGCGTCGACACCGATGCCGTTTGGTCCGTATCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.396	96.528	0.535
pilD	Vibrio campbellii strain DS40M4	51.613	96.875	0.5
pilD	Neisseria gonorrhoeae MS11	51.111	93.75	0.479
pilD	Acinetobacter baumannii D1279779	48.718	94.792	0.462
pilD	Acinetobacter nosocomialis M2	48.718	94.792	0.462