Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   U0449_RS05355 Genome accession   NZ_CP139862
Coordinates   1057712..1059010 (+) Length   432 a.a.
NCBI ID   WP_000867609.1    Uniprot ID   A0A0T8HBK0
Organism   Streptococcus pneumoniae strain 05H0020-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1052712..1064010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0449_RS05325 tsf 1053158..1054198 (+) 1041 WP_061543497.1 translation elongation factor Ts -
  U0449_RS05330 tnpB 1054444..1054767 (+) 324 WP_000586641.1 IS66 family insertion sequence element accessory protein TnpB -
  U0449_RS05335 - 1054922..1055230 (-) 309 Protein_1053 transposase -
  U0449_RS05340 - 1055203..1055741 (+) 539 Protein_1054 transposase -
  U0449_RS05345 cysK 1055816..1056736 (-) 921 WP_000029866.1 cysteine synthase A -
  U0449_RS05350 - 1057021..1057656 (-) 636 WP_000395739.1 YigZ family protein -
  U0449_RS05355 comFA/cflA 1057712..1059010 (+) 1299 WP_000867609.1 DEAD/DEAH box helicase Machinery gene
  U0449_RS05360 comFC/cflB 1059007..1059669 (+) 663 WP_000649964.1 ComF family protein Machinery gene
  U0449_RS05365 hpf 1059749..1060297 (+) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  U0449_RS05370 - 1060433..1062406 (+) 1974 WP_050205401.1 DHH family phosphoesterase -
  U0449_RS05375 rplI 1062403..1062855 (+) 453 WP_050114792.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49505.44 Da        Isoelectric Point: 8.5992

>NTDB_id=911484 U0449_RS05355 WP_000867609.1 1057712..1059010(+) (comFA/cflA) [Streptococcus pneumoniae strain 05H0020-2]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=911484 U0449_RS05355 WP_000867609.1 1057712..1059010(+) (comFA/cflA) [Streptococcus pneumoniae strain 05H0020-2]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTATCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T8HBK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae TIGR4

99.306

100

0.993
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389