Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HX0037_RS16475 Genome accession   NZ_CP139782
Coordinates   3337038..3337652 (+) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus amyloliquefaciens strain HX0037     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3332038..3342652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HX0037_RS16440 aroE 3332725..3333567 (+) 843 WP_094247716.1 shikimate dehydrogenase -
  HX0037_RS16445 yhbY 3333561..3333851 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  HX0037_RS16450 - 3333862..3334431 (+) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  HX0037_RS16455 yqeK 3334421..3334981 (+) 561 WP_032856887.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HX0037_RS16460 rsfS 3334999..3335355 (+) 357 WP_003152864.1 ribosome silencing factor -
  HX0037_RS16465 - 3335352..3336089 (+) 738 WP_031378935.1 class I SAM-dependent DNA methyltransferase -
  HX0037_RS16470 comER 3336158..3336979 (-) 822 WP_014305449.1 late competence protein ComER -
  HX0037_RS16475 comEA 3337038..3337652 (+) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  HX0037_RS16480 - 3337719..3338288 (+) 570 WP_003152868.1 ComE operon protein 2 -
  HX0037_RS16485 comEC 3338289..3340640 (+) 2352 WP_094247717.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HX0037_RS16490 - 3340659..3340793 (-) 135 WP_003152870.1 YqzM family protein -
  HX0037_RS16495 - 3340834..3340988 (+) 155 Protein_3195 hypothetical protein -
  HX0037_RS16500 holA 3341028..3342069 (+) 1042 Protein_3196 DNA polymerase III subunit delta -
  HX0037_RS16505 rpsT 3342086..3342352 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=910997 HX0037_RS16475 WP_013352942.1 3337038..3337652(+) (comEA) [Bacillus amyloliquefaciens strain HX0037]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=910997 HX0037_RS16475 WP_013352942.1 3337038..3337652(+) (comEA) [Bacillus amyloliquefaciens strain HX0037]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363