Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABOX98_RS29920 Genome accession   NZ_CP158570
Coordinates   6313566..6314189 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain SRPA1308     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6308566..6319189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABOX98_RS29900 (ABOX98_29900) aroB 6309161..6310267 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  ABOX98_RS29905 (ABOX98_29905) aroK 6310317..6310835 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ABOX98_RS29910 (ABOX98_29910) pilQ 6310847..6312991 (-) 2145 WP_031686163.1 type 4a pilus secretin PilQ Machinery gene
  ABOX98_RS29915 (ABOX98_29915) comL/pilP 6313045..6313569 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ABOX98_RS29920 (ABOX98_29920) pilO 6313566..6314189 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABOX98_RS29925 (ABOX98_29925) pilN 6314186..6314782 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ABOX98_RS29930 (ABOX98_29930) pilM 6314782..6315846 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ABOX98_RS29935 (ABOX98_29935) - 6316031..6318502 (+) 2472 WP_031686164.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=910292 ABOX98_RS29920 WP_003103268.1 6313566..6314189(-) (pilO) [Pseudomonas aeruginosa strain SRPA1308]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=910292 ABOX98_RS29920 WP_003103268.1 6313566..6314189(-) (pilO) [Pseudomonas aeruginosa strain SRPA1308]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401