Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SD437_RS12765 Genome accession   NZ_CP139565
Coordinates   2559878..2560492 (-) Length   204 a.a.
NCBI ID   WP_017418180.1    Uniprot ID   -
Organism   Bacillus velezensis strain NCCP     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554878..2565492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD437_RS12735 rpsT 2555181..2555447 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  SD437_RS12740 holA 2555464..2556505 (-) 1042 Protein_2463 DNA polymerase III subunit delta -
  SD437_RS12745 - 2556545..2556696 (-) 152 Protein_2464 hypothetical protein -
  SD437_RS12750 - 2556737..2556871 (+) 135 WP_003152870.1 YqzM family protein -
  SD437_RS12755 comEC 2556890..2559241 (-) 2352 WP_017418179.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SD437_RS12760 - 2559242..2559811 (-) 570 WP_003152868.1 ComE operon protein 2 -
  SD437_RS12765 comEA 2559878..2560492 (-) 615 WP_017418180.1 helix-hairpin-helix domain-containing protein Machinery gene
  SD437_RS12770 comER 2560551..2561372 (+) 822 WP_014305449.1 late competence protein ComER -
  SD437_RS12775 - 2561441..2562178 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  SD437_RS12780 rsfS 2562175..2562531 (-) 357 WP_003152864.1 ribosome silencing factor -
  SD437_RS12785 yqeK 2562528..2563109 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SD437_RS12790 - 2563099..2563668 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  SD437_RS12795 yhbY 2563679..2563969 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  SD437_RS12800 aroE 2563963..2564805 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 5.4841

>NTDB_id=910232 SD437_RS12765 WP_017418180.1 2559878..2560492(-) (comEA) [Bacillus velezensis strain NCCP]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=910232 SD437_RS12765 WP_017418180.1 2559878..2560492(-) (comEA) [Bacillus velezensis strain NCCP]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363