Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABS957_RS31200 Genome accession   NZ_CP158482
Coordinates   6542884..6543507 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 1485138     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6537884..6548507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS957_RS31180 (ABS957_31175) aroB 6538479..6539585 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  ABS957_RS31185 (ABS957_31180) aroK 6539635..6540153 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ABS957_RS31190 (ABS957_31185) pilQ 6540165..6542309 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  ABS957_RS31195 (ABS957_31190) comL/pilP 6542363..6542887 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ABS957_RS31200 (ABS957_31195) pilO 6542884..6543507 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABS957_RS31205 (ABS957_31200) pilN 6543504..6544100 (-) 597 Protein_6167 type 4a pilus biogenesis protein PilN -
  ABS957_RS31210 (ABS957_31205) pilM 6544100..6545164 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ABS957_RS31215 (ABS957_31210) - 6545349..6547817 (+) 2469 WP_025297613.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=910029 ABS957_RS31200 WP_003103268.1 6542884..6543507(-) (pilO) [Pseudomonas aeruginosa strain 1485138]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=910029 ABS957_RS31200 WP_003103268.1 6542884..6543507(-) (pilO) [Pseudomonas aeruginosa strain 1485138]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401