Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   L6431_RS09960 Genome accession   NZ_AP025273
Coordinates   2105301..2106893 (-) Length   530 a.a.
NCBI ID   WP_237046412.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain MRCP2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 2100301..2111893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6431_RS09925 - 2100585..2101157 (-) 573 WP_237046407.1 PilW family protein -
  L6431_RS09930 pilV 2101154..2101687 (-) 534 WP_237046408.1 type IV pilus modification protein PilV -
  L6431_RS09935 - 2101701..2102150 (-) 450 WP_272878770.1 GspH/FimT family pseudopilin -
  L6431_RS09945 - 2102249..2102764 (-) 516 WP_237046409.1 GspH/FimT family pseudopilin -
  L6431_RS09950 thiO 2102825..2103934 (+) 1110 WP_237046410.1 glycine oxidase ThiO -
  L6431_RS09955 pilR 2103958..2105298 (-) 1341 WP_237046411.1 sigma-54-dependent transcriptional regulator Regulator
  L6431_RS09960 pilS 2105301..2106893 (-) 1593 WP_237046412.1 sensor histidine kinase Regulator
  L6431_RS09965 - 2106890..2107114 (-) 225 WP_237046413.1 PP0621 family protein -
  L6431_RS09970 - 2107236..2108258 (-) 1023 WP_142136537.1 outer membrane protein assembly factor BamD -
  L6431_RS09975 rluD 2108406..2109377 (+) 972 WP_237046414.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  L6431_RS09980 pgeF 2109361..2110098 (+) 738 WP_237046415.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59105.39 Da        Isoelectric Point: 7.6073

>NTDB_id=90912 L6431_RS09960 WP_237046412.1 2105301..2106893(-) (pilS) [Aquipseudomonas alcaligenes strain MRCP2]
MSTEQLTLDGVPGRRILRLYNLYRVIIGLALVLLISSDLDDQLLELAHAELFRYGSWTYLILNVLVAVLLQRPSHLAQVF
SLALLDVLLLCTLFFAAGGTPSGIGNLLIVAVAIANILLRGRIGLLIAAVAAIGMLALTFYLSLLRNTPAPQFVQAGALG
ALCFAAAIFLQGLASRLQQSEFLAEQRAVDVASLEALNSLILQRMRTGILVLDKQHRVLLANQGALSMLGRSKLEHKLLD
PHCPELVQRLQQWQHNPTLRPQSLQAIADGPLLQPSFIPLQRSGEQHTLIFLDDISQVTQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLEEPDRRLAQIIQDHSRRMNTVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSARPD
QTLHLETQSGSLQTRMDPHQLTQILSNLVQNGLRYSAQKHQLGQVWLKLYRDPESDLPTLEVLDDGPGVPVDQMQHIFEP
FYTTDNKGTGLGLYISRELCESNQARLDYKLREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=90912 L6431_RS09960 WP_237046412.1 2105301..2106893(-) (pilS) [Aquipseudomonas alcaligenes strain MRCP2]
GTGAGCACCGAGCAGCTGACCCTCGACGGCGTCCCCGGCAGACGCATACTGCGCCTGTACAACCTCTATCGGGTGATCAT
CGGCCTGGCGCTGGTGCTGCTGATTTCCAGCGACCTGGACGACCAGTTGCTGGAGCTGGCACACGCCGAGCTGTTCCGCT
ATGGCAGCTGGACCTACCTGATACTCAACGTGCTGGTCGCCGTACTGCTGCAGCGCCCGAGCCACCTGGCCCAGGTCTTC
AGCCTGGCACTGCTCGACGTGCTGCTGCTGTGTACCCTGTTCTTCGCCGCAGGCGGTACCCCGAGCGGCATCGGCAACCT
GCTGATAGTCGCGGTGGCCATCGCCAACATCCTGTTGCGCGGCCGTATCGGCCTGCTGATCGCCGCCGTCGCCGCCATCG
GCATGCTGGCCCTAACCTTCTATCTCAGCCTGCTGCGCAACACGCCAGCTCCGCAGTTCGTCCAGGCAGGCGCCCTCGGC
GCCCTGTGCTTCGCCGCCGCGATCTTCCTCCAGGGCCTGGCCAGCCGACTGCAGCAGAGCGAGTTCCTGGCCGAACAGCG
TGCCGTCGATGTTGCCAGCCTGGAGGCACTCAACTCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTCCTGGACA
AGCAGCATCGCGTGTTGCTGGCCAATCAGGGCGCCCTCAGCATGCTCGGGCGCAGCAAGCTGGAGCACAAACTGCTCGAC
CCGCACTGCCCGGAGCTGGTCCAGCGCCTGCAACAGTGGCAGCACAACCCGACCCTGCGCCCGCAGAGCCTGCAGGCCAT
AGCGGACGGCCCGTTGTTGCAGCCGAGCTTCATCCCGCTGCAACGCAGCGGCGAACAGCACACCCTGATCTTCCTCGACG
ACATCTCGCAGGTTACCCAGCAGGCCCAGCAGCTCAAGCTGGCCTCCCTGGGGCGCCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCGGAGGACCTGGAGGAGCCGGATCGGCG
CCTGGCGCAGATCATCCAGGACCATTCGCGGCGCATGAATACGGTGATCGAAAACGTCCTGCAACTGTCGCGCAGGCGTC
AGGCCGAGCCTCAGCTGCTTGACCTGAAATACTGGCTGCACCGCTTCGCCAGCGAGTTCCGCAGTTCCGCACGGCCGGAC
CAGACACTGCACCTGGAGACCCAGAGCGGCAGCCTGCAGACCCGCATGGACCCGCACCAGCTGACGCAGATTCTGAGCAA
TCTGGTGCAGAACGGCCTGCGCTACAGCGCGCAGAAGCACCAGCTGGGACAGGTCTGGCTCAAGCTCTATCGCGATCCGG
AGAGCGACCTGCCGACACTCGAGGTGCTGGATGACGGCCCCGGCGTACCGGTCGACCAGATGCAGCACATCTTCGAACCC
TTCTACACCACGGACAACAAGGGCACCGGCCTCGGCCTGTATATTTCCCGCGAACTGTGCGAGAGCAATCAAGCACGCCT
CGATTACAAACTGCGCGAGGGTGGCGGCAGTTGTTTCCGCATCACCTTCGCCCACCCACGCAAGCTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.415

100

0.664

Multiple sequence alignment