Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   SJN48_RS01550 Genome accession   NZ_CP139212
Coordinates   327100..327852 (+) Length   250 a.a.
NCBI ID   WP_060464993.1    Uniprot ID   A0A1E2YH36
Organism   Acinetobacter pittii strain WWTP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322100..332852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SJN48_RS01535 (SJN48_01540) ponA 322681..325242 (-) 2562 WP_130121783.1 penicillin-binding protein PBP1a -
  SJN48_RS01540 (SJN48_01545) pilM 325404..326462 (+) 1059 WP_033848910.1 pilus assembly protein PilM Machinery gene
  SJN48_RS01545 (SJN48_01550) pilN 326462..327103 (+) 642 WP_033848908.1 PilN domain-containing protein Machinery gene
  SJN48_RS01550 (SJN48_01555) pilO 327100..327852 (+) 753 WP_060464993.1 type 4a pilus biogenesis protein PilO Machinery gene
  SJN48_RS01555 (SJN48_01560) pilP 327863..328390 (+) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  SJN48_RS01560 (SJN48_01565) pilQ 328453..330579 (+) 2127 WP_130121781.1 type IV pilus secretin PilQ Machinery gene
  SJN48_RS01565 (SJN48_01570) aroK 330591..331160 (+) 570 WP_002116987.1 shikimate kinase AroK -
  SJN48_RS01570 (SJN48_01575) aroB 331189..332271 (+) 1083 WP_005067096.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28241.10 Da        Isoelectric Point: 4.8782

>NTDB_id=908094 SJN48_RS01550 WP_060464993.1 327100..327852(+) (pilO) [Acinetobacter pittii strain WWTP]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=908094 SJN48_RS01550 WP_060464993.1 327100..327852(+) (pilO) [Acinetobacter pittii strain WWTP]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGTGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCCGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGGATTGTGAC
GATGCATGATTTTATTGTTGAGGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCCATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E2YH36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668