NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	SH598_RS12560	Genome accession	NZ_CP139086
Coordinates	2606498..2607112 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain YN-2A
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2601498..2612112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SH598_RS12530 (SH598_12530)	rpsT	2601801..2602067 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
SH598_RS12535 (SH598_12535)	holA	2602084..2603125 (-)	1042	Protein_2426	DNA polymerase III subunit delta	-
SH598_RS12540 (SH598_12540)	-	2603165..2603316 (-)	152	Protein_2427	hypothetical protein	-
SH598_RS12545 (SH598_12545)	-	2603357..2603491 (+)	135	WP_003152870.1	YqzM family protein	-
SH598_RS12550 (SH598_12550)	comEC	2603510..2605861 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SH598_RS12555 (SH598_12555)	-	2605862..2606431 (-)	570	WP_003152868.1	ComE operon protein 2	-
SH598_RS12560 (SH598_12560)	comEA	2606498..2607112 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
SH598_RS12565 (SH598_12565)	comER	2607171..2607992 (+)	822	WP_039254409.1	late competence protein ComER	-
SH598_RS12570 (SH598_12570)	-	2608061..2608798 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
SH598_RS12575 (SH598_12575)	rsfS	2608795..2609151 (-)	357	WP_007408260.1	ribosome silencing factor	-
SH598_RS12580 (SH598_12580)	yqeK	2609169..2609729 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SH598_RS12585 (SH598_12585)	-	2609719..2610288 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
SH598_RS12590 (SH598_12590)	yhbY	2610299..2610589 (-)	291	WP_320562532.1	ribosome assembly RNA-binding protein YhbY	-
SH598_RS12595 (SH598_12595)	aroE	2610583..2611425 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=907176 SH598_RS12560 WP_015240245.1 2606498..2607112(-) (comEA) [Bacillus velezensis strain YN-2A]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=907176 SH598_RS12560 WP_015240245.1 2606498..2607112(-) (comEA) [Bacillus velezensis strain YN-2A]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0907176