Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB37_RS02945 Genome accession   NZ_CP138954
Coordinates   566592..568385 (+) Length   597 a.a.
NCBI ID   WP_413404610.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9510     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561592..573385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB37_RS02915 murB 562233..563165 (+) 933 WP_413404599.1 UDP-N-acetylmuramate dehydrogenase -
  SIB37_RS02920 - 563190..563531 (+) 342 WP_413404601.1 YbaB/EbfC family nucleoid-associated protein -
  SIB37_RS02925 rsgA 563506..564387 (-) 882 WP_413404603.1 ribosome small subunit-dependent GTPase A -
  SIB37_RS02930 - 564407..564649 (-) 243 WP_413404605.1 sulfurtransferase TusA family protein -
  SIB37_RS02935 dnaJ 564652..565782 (-) 1131 WP_413404608.1 molecular chaperone DnaJ -
  SIB37_RS02940 grpE 565825..566562 (-) 738 WP_413406182.1 nucleotide exchange factor GrpE -
  SIB37_RS02945 pilB/pilB1 566592..568385 (+) 1794 WP_413404610.1 GspE/PulE family protein Machinery gene
  SIB37_RS02950 pilT 568396..569472 (+) 1077 WP_413404612.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB37_RS02955 - 569476..570900 (+) 1425 WP_413404614.1 type II secretion system F family protein -
  SIB37_RS02960 - 570914..572203 (-) 1290 WP_413404616.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB37_RS02965 msrB 572133..572642 (-) 510 WP_413404618.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65246.39 Da        Isoelectric Point: 4.9713

>NTDB_id=906204 SIB37_RS02945 WP_413404610.1 566592..568385(+) (pilB/pilB1) [Synechococcus sp. MIT S9510]
MLIGEAVLPQAGEARTFLRIEAVSDDRRHLELELLLQRAIPGDFGIADQPVLLEELATMGFDPARHAALAESLRKLLDNN
LPLSDEALPLKPPITETPPRHIEVLPGPMTSVSNAAEAETVAETATSYLRDFNVNGVLEADPDEKDADSSKPADLESGLN
DAERSPVIDLVDRILMQALELGASDIHVEPQQAGLQLRFRQDGVLQSQIEPLPSRMVPAVTSRFKIMAELDIAERRLPQD
GRIRRRFQNRTVDFRVNSLPSRYGEKIVLRLLDSSATQLGLDKLISNADALNLVRTLGSKPFGMILVTGPTGSGKSTTLY
SLLAERNEPGINISTIEDPIEYTLPGITQCQVNREKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVL
TTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCSTCRIAYRPDPQELGRFGLMASNEANVSFFRANHHEGQ
ANPCPHCQGSGYKGRVGVYEVLRMNEALAAAVAKGATTDMVRQLALEGGMKTLLGYSLDLVREGHTTLEEVGRMVLTDAG
LESERRARALSTLTCHGCGGGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=906204 SIB37_RS02945 WP_413404610.1 566592..568385(+) (pilB/pilB1) [Synechococcus sp. MIT S9510]
ATGTTGATAGGTGAGGCAGTCCTCCCACAAGCGGGGGAAGCCCGCACCTTTCTGCGAATTGAAGCCGTGAGCGACGACCG
TCGGCATCTGGAACTGGAACTTCTGCTGCAGCGAGCCATTCCAGGCGATTTCGGCATCGCTGATCAACCTGTTCTGCTCG
AGGAACTGGCGACGATGGGATTTGATCCTGCTCGTCACGCTGCGCTCGCCGAAAGCCTGAGAAAGCTGCTCGACAACAAC
CTCCCGTTGAGCGATGAAGCACTCCCCCTCAAACCACCCATCACGGAAACACCGCCAAGACATATCGAGGTCTTGCCGGG
ACCCATGACCTCAGTTTCAAACGCTGCAGAGGCCGAAACCGTTGCCGAGACGGCTACCTCCTATCTGAGGGATTTCAACG
TCAATGGGGTGCTGGAAGCTGATCCCGACGAGAAGGATGCCGACTCCTCAAAACCCGCGGATCTTGAATCGGGGCTCAAC
GATGCGGAACGCTCACCGGTGATCGATCTGGTGGATCGCATCCTGATGCAGGCCCTCGAACTGGGTGCCAGCGATATCCA
TGTGGAGCCTCAACAGGCCGGACTCCAGCTGCGCTTTCGTCAGGACGGGGTGCTGCAGAGCCAGATCGAACCTCTGCCCA
GTCGGATGGTGCCTGCGGTGACCTCCAGATTCAAGATCATGGCGGAACTGGACATCGCCGAACGCCGGCTTCCCCAGGAC
GGCCGCATTCGCCGCCGCTTCCAGAACAGGACAGTGGATTTCAGGGTCAACAGTCTGCCGAGCCGCTATGGGGAAAAAAT
CGTTCTGCGGCTTCTGGACAGCTCGGCCACCCAGCTGGGTCTCGACAAACTCATTTCCAATGCTGACGCCCTGAATCTGG
TGCGCACCTTGGGATCCAAGCCCTTCGGAATGATCCTGGTCACAGGACCGACTGGATCCGGTAAGTCGACAACGCTGTAT
TCCCTGCTGGCGGAGCGCAACGAACCAGGAATCAACATCTCCACCATTGAGGACCCCATCGAATACACGCTGCCAGGCAT
CACCCAGTGTCAGGTGAATAGGGAGAAGGGATTTGACTTCAGCCAGGCACTGAGGGCCTTCATGCGCCAGGACCCGGACG
TGCTGCTGGTGGGCGAAACGCGCGATCTGGAAACCGCGAAAACAGCCATCGAAGCCGCACTCACCGGTCACCTGGTGCTG
ACCACCCTTCACTGCAACGATGCTCCCAGCGCCATCGCCCGTCTCGATGAGATGGGTGTGGAGCCTTTCATGGTCAGCGC
CTCACTGATCGGGATCGTCTCCCAGCGTCTGCTCCGACGGGTCTGCTCGACTTGCAGGATCGCCTACCGGCCTGATCCCC
AAGAACTGGGTCGTTTCGGCCTGATGGCGAGCAATGAAGCGAACGTGAGCTTTTTCAGAGCCAACCACCATGAAGGGCAG
GCCAACCCATGCCCCCATTGCCAGGGCAGTGGTTACAAGGGACGCGTGGGGGTCTACGAAGTGCTGCGCATGAATGAAGC
TCTCGCGGCCGCCGTAGCCAAGGGCGCCACCACAGACATGGTGCGACAGCTCGCTCTGGAGGGAGGGATGAAAACCTTGC
TGGGTTACAGCCTTGACCTGGTGAGGGAGGGACACACGACCCTGGAGGAAGTGGGCAGGATGGTTCTCACAGACGCCGGG
CTGGAATCGGAACGACGCGCACGCGCTCTCAGCACCCTCACCTGCCATGGCTGTGGTGGCGGACTCCAGGAAGGTTGGCT
GGAATGTCCCTACTGCCTGACACCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(174-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.46

82.58

0.508