Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG/mmsA   Type   Machinery gene
Locus tag   ABLU27_RS03750 Genome accession   NZ_CP157500
Coordinates   655860..657860 (-) Length   666 a.a.
NCBI ID   WP_003131242.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 650860..662860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU27_RS03725 (ABLU27_03725) - 651332..651946 (+) 615 WP_010906406.1 TetR/AcrR family transcriptional regulator -
  ABLU27_RS03730 (ABLU27_03730) - 652025..652753 (-) 729 WP_031296592.1 pseudouridine synthase -
  ABLU27_RS03735 (ABLU27_03735) - 652849..653484 (-) 636 WP_003132036.1 hypothetical protein -
  ABLU27_RS03740 (ABLU27_03740) mnmE 653696..655063 (+) 1368 WP_003131240.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ABLU27_RS03745 (ABLU27_03745) - 655114..655860 (-) 747 Protein_640 hypothetical protein -
  ABLU27_RS03750 (ABLU27_03750) recG/mmsA 655860..657860 (-) 2001 WP_003131242.1 ATP-dependent DNA helicase RecG Machinery gene
  ABLU27_RS03755 (ABLU27_03755) - 657998..658342 (-) 345 WP_010906411.1 zinc ribbon domain-containing protein YjdM -
  ABLU27_RS03760 (ABLU27_03760) gap 658412..659422 (-) 1011 WP_406835011.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  ABLU27_RS03765 (ABLU27_03765) - 659702..660223 (-) 522 WP_003131246.1 DUF536 domain-containing protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74860.59 Da        Isoelectric Point: 5.8208

>NTDB_id=906052 ABLU27_RS03750 WP_003131242.1 655860..657860(-) (recG/mmsA) [Lactococcus lactis strain 2B-9]
MKLTDSVQFLKGVGPKSVENFHKLGIFTVQDLLLYFPFRYEDFASRSVFELVDGEKATIIGTVVTPANVQYYGFKRNRLS
FKIKEGEAVVAVSFFNQPYLADKVEVGKEIAVYGKWEQAKQQLMGMKVVAQVDDGLEPVYHLTAGMKQSQLVKVIHQVFE
SDLLSELPENLPEFLIKKYRLMNRQEAVLAMHFPEDMEAHKQALRRVKFEELFMFQLKLQALKNKEKSGRAGLEVVFEQG
EIDQKISELPFELTGAQLSALSEILTDMKSPYHMNRLLQGDVGSGKTVVASLAMYAACLANFQAAIMVPTEILARQHFAN
LKALFPELQISLLVSGLKVAERREILADLVSGHTHMVVGTHALIQEGVDFYNLGLVITDEQHRFGVNQRKILREKGQNPD
VLMMTATPIPRTLAITAFGDMDVSIIDELPKGRQPITTRWVKHEQFSEVLSWIKAEVAKDSQVYFISPLIEESETLDLKN
AEALFAELNEYFGLFAKIGLLHGKMKNDEKDQIMQEFKAKKLDILVSTTVIEVGVDVPNATIMVIMDADRFGLSQLHQLR
GRVGRGTKKSYAILVANPKSDSGKQRMKIMTETQNGFILAEEDLKMRGSGEIFGLRQSGLPEFIAADLVNDYNILEVARK
EAVEIFKNADEFPDLIAQVNVDGGFD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=906052 ABLU27_RS03750 WP_003131242.1 655860..657860(-) (recG/mmsA) [Lactococcus lactis strain 2B-9]
ATGAAATTAACAGATTCAGTGCAATTTTTAAAAGGTGTGGGACCGAAATCTGTGGAAAATTTCCATAAGTTAGGAATTTT
CACGGTTCAAGATTTATTGCTTTATTTCCCTTTTCGTTATGAAGATTTCGCAAGTCGTTCGGTTTTTGAACTTGTTGACG
GAGAAAAAGCAACAATTATTGGAACGGTAGTGACACCAGCTAATGTCCAGTATTACGGATTTAAACGTAATCGACTTTCA
TTTAAAATTAAAGAAGGTGAGGCCGTGGTTGCTGTCAGCTTTTTTAATCAACCTTATTTGGCTGACAAAGTGGAAGTTGG
TAAGGAAATTGCAGTTTACGGAAAATGGGAGCAGGCAAAGCAACAACTGATGGGGATGAAGGTTGTGGCTCAGGTGGACG
ATGGCTTAGAACCTGTTTATCATTTGACAGCGGGTATGAAACAAAGTCAATTGGTCAAAGTAATTCATCAGGTTTTTGAA
TCAGACCTTCTATCAGAATTACCTGAGAACTTACCAGAATTTTTGATTAAAAAATATCGTCTGATGAACCGGCAAGAAGC
CGTTTTGGCTATGCATTTTCCTGAAGATATGGAAGCTCATAAGCAGGCTTTACGTCGTGTAAAATTTGAAGAATTATTCA
TGTTTCAGTTGAAATTACAGGCCTTAAAAAATAAAGAAAAATCAGGCCGAGCAGGATTAGAAGTTGTTTTTGAGCAAGGA
GAAATTGATCAGAAGATTTCTGAATTACCTTTTGAACTGACAGGTGCTCAATTAAGCGCTTTGTCAGAGATTCTGACGGA
TATGAAATCTCCTTATCACATGAATCGACTTTTGCAAGGTGATGTGGGTTCTGGTAAAACAGTTGTTGCTTCCCTTGCCA
TGTACGCGGCTTGTTTGGCGAATTTTCAAGCAGCAATTATGGTACCAACGGAAATCCTTGCCCGTCAGCATTTTGCAAAC
TTGAAAGCTCTTTTTCCAGAATTGCAAATTTCACTTTTGGTTTCGGGTTTGAAAGTGGCAGAGCGCCGCGAGATTTTGGC
TGATTTAGTTAGTGGTCATACACATATGGTAGTTGGAACTCATGCGCTAATTCAAGAAGGGGTTGATTTTTATAATTTGG
GTTTGGTGATTACAGACGAACAACACCGTTTCGGAGTCAATCAACGAAAAATTTTACGAGAAAAAGGGCAAAATCCAGAT
GTTCTAATGATGACAGCTACACCCATTCCAAGAACGCTTGCGATTACGGCTTTTGGGGATATGGATGTTTCAATTATTGA
TGAACTACCCAAGGGACGTCAACCCATTACTACAAGGTGGGTCAAACATGAACAATTTTCCGAAGTTTTAAGTTGGATAA
AAGCAGAGGTTGCTAAAGATTCACAGGTTTATTTTATTTCACCTTTAATTGAGGAAAGTGAAACGTTGGACCTGAAAAAC
GCAGAAGCTCTTTTTGCGGAATTAAATGAATACTTTGGATTATTTGCCAAAATTGGTCTTTTACATGGAAAAATGAAAAA
TGATGAAAAAGACCAAATCATGCAAGAATTTAAGGCTAAAAAATTAGATATTTTGGTTTCAACAACAGTGATTGAAGTGG
GAGTTGATGTTCCTAATGCTACCATTATGGTGATTATGGATGCTGACCGCTTTGGACTTTCGCAGCTTCACCAATTGCGT
GGTCGTGTTGGTCGGGGAACAAAAAAATCTTATGCCATTTTAGTAGCAAATCCTAAGTCCGATTCAGGAAAACAACGGAT
GAAAATTATGACAGAAACACAAAATGGTTTTATTTTAGCCGAAGAAGATTTGAAAATGCGTGGTTCTGGAGAGATTTTTG
GACTTCGTCAGTCAGGACTTCCAGAATTTATTGCGGCTGATTTAGTCAATGATTATAATATTTTAGAAGTGGCTAGAAAA
GAAGCAGTTGAAATTTTTAAAAATGCAGATGAGTTTCCTGATTTGATTGCTCAAGTTAATGTTGACGGGGGCTTTGATTG
A

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(456-566)

RecG wedge domain (RecG_wedge)

(7-157)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(593-651)

DEAD/DEAH box helicase (DEAD)

(257-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG/mmsA Streptococcus pneumoniae R6

66.047

96.847

0.64
  recG/mmsA Streptococcus pneumoniae R36A

66.047

96.847

0.64
  recG Bacillus subtilis subsp. subtilis str. 168

50.698

96.847

0.491
  recG Neisseria meningitidis strain C311

40.181

99.399

0.399