Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BSG8_RS12655 Genome accession   NZ_AP025224
Coordinates   2330806..2331423 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. natto strain G8     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2325806..2336423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSG8_RS12625 (BSG8_25050) rpsT 2326108..2326374 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  BSG8_RS12630 (BSG8_25060) holA 2326389..2327432 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  BSG8_RS12635 - 2327472..2327621 (-) 150 WP_003229985.1 hypothetical protein -
  BSG8_RS12640 (BSG8_25070) yqzM 2327662..2327796 (+) 135 WP_003229983.1 YqzM family protein -
  BSG8_RS12645 comEC 2327836..2330166 (-) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BSG8_RS12650 (BSG8_25100) comEB 2330170..2330739 (-) 570 WP_003229978.1 ComE operon protein 2 -
  BSG8_RS12655 (BSG8_25110) comEA 2330806..2331423 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  BSG8_RS12660 (BSG8_25120) comER 2331507..2332328 (+) 822 WP_014480313.1 late competence protein ComER -
  BSG8_RS12665 (BSG8_25130) yqeM 2332394..2333137 (-) 744 WP_014480314.1 class I SAM-dependent methyltransferase -
  BSG8_RS12670 (BSG8_25140) rsfS 2333134..2333490 (-) 357 WP_014480315.1 ribosome silencing factor -
  BSG8_RS12675 (BSG8_25150) yqeK 2333508..2334068 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BSG8_RS12680 (BSG8_25160) nadD 2334058..2334627 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  BSG8_RS12685 (BSG8_25170) yhbY 2334639..2334929 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BSG8_RS12690 (BSG8_25180) aroE 2334923..2335765 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=90573 BSG8_RS12655 WP_014480312.1 2330806..2331423(-) (comEA) [Bacillus subtilis subsp. natto strain G8]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=90573 BSG8_RS12655 WP_014480312.1 2330806..2331423(-) (comEA) [Bacillus subtilis subsp. natto strain G8]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment