Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SBP12_RS12640 Genome accession   NZ_CP138634
Coordinates   2572499..2573113 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain A5     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2567499..2578113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBP12_RS12610 (SBP12_12610) rpsT 2567802..2568068 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  SBP12_RS12615 (SBP12_12615) holA 2568085..2569126 (-) 1042 Protein_2438 DNA polymerase III subunit delta -
  SBP12_RS12620 (SBP12_12620) - 2569166..2569317 (-) 152 Protein_2439 hypothetical protein -
  SBP12_RS12625 (SBP12_12625) - 2569358..2569492 (+) 135 WP_003152870.1 YqzM family protein -
  SBP12_RS12630 (SBP12_12630) comEC 2569511..2571862 (-) 2352 WP_039254415.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SBP12_RS12635 (SBP12_12635) - 2571863..2572432 (-) 570 WP_003152868.1 ComE operon protein 2 -
  SBP12_RS12640 (SBP12_12640) comEA 2572499..2573113 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  SBP12_RS12645 (SBP12_12645) comER 2573172..2573993 (+) 822 WP_039254409.1 late competence protein ComER -
  SBP12_RS12650 (SBP12_12650) - 2574062..2574799 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  SBP12_RS12655 (SBP12_12655) rsfS 2574796..2575152 (-) 357 WP_007408260.1 ribosome silencing factor -
  SBP12_RS12660 (SBP12_12660) yqeK 2575170..2575730 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SBP12_RS12665 (SBP12_12665) - 2575720..2576289 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  SBP12_RS12670 (SBP12_12670) yhbY 2576300..2576590 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  SBP12_RS12675 (SBP12_12675) aroE 2576584..2577426 (-) 843 WP_039254404.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=903862 SBP12_RS12640 WP_015240245.1 2572499..2573113(-) (comEA) [Bacillus velezensis strain A5]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=903862 SBP12_RS12640 WP_015240245.1 2572499..2573113(-) (comEA) [Bacillus velezensis strain A5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363