Detailed information NTG0090318

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CC22TP_RS07530
• Genome accession: NZ_AP025177
• Coordinates: 1573898..1574395 (-)
• Length: 165 a.a.
• NCBI ID: WP_029697681.1
• Uniprot ID: A0AAE2ZZ83
• Organism: Staphylococcus aureus strain TPS6281
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1568898..1579395

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CC22TP_RS07505 (TPS6281TP_14350)	gcvPB	1569060..1570532 (-)	1473	WP_000202191.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
CC22TP_RS07510 (TPS6281TP_14360)	gcvPA	1570525..1571871 (-)	1347	WP_000019688.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
CC22TP_RS07515 (TPS6281TP_14370)	gcvT	1571891..1572982 (-)	1092	WP_000093355.1	glycine cleavage system aminomethyltransferase GcvT	-
CC22TP_RS07520 (TPS6281TP_14380)	-	1573141..1573665 (-)	525	WP_001015122.1	shikimate kinase	-
CC22TP_RS07525	-	1573655..1573801 (-)	147	WP_001789879.1	hypothetical protein	-
CC22TP_RS07530 (TPS6281TP_14390)	comGF	1573898..1574395 (-)	498	WP_029697681.1	competence type IV pilus minor pilin ComGF	Machinery gene
CC22TP_RS07535 (TPS6281TP_14400)	comGE	1574313..1574612 (-)	300	WP_217654599.1	competence protein ComGE	Machinery gene
CC22TP_RS07540 (TPS6281TP_14410)	comGD	1574599..1575045 (-)	447	WP_075583651.1	competence type IV pilus minor pilin ComGD	Machinery gene
CC22TP_RS07545 (TPS6281TP_14420)	comGC	1575023..1575334 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
CC22TP_RS07550 (TPS6281TP_14430)	comGB	1575348..1576418 (-)	1071	WP_000776403.1	competence type IV pilus assembly protein ComGB	Machinery gene
CC22TP_RS07555 (TPS6281TP_14440)	comGA	1576390..1577364 (-)	975	WP_000697215.1	competence type IV pilus ATPase ComGA	Machinery gene
CC22TP_RS07560 (TPS6281TP_14450)	-	1577416..1578039 (-)	624	WP_001223006.1	MBL fold metallo-hydrolase	-
CC22TP_RS07565 (TPS6281TP_14460)	-	1578036..1578365 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
CC22TP_RS07570 (TPS6281TP_14470)	-	1578365..1579351 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19211.94 Da
• Isoelectric Point: 10.2436

>NTDB_id=90318 CC22TP_RS07530 WP_029697681.1 1573898..1574395(-) (comGF) [Staphylococcus aureus strain TPS6281]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFRGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=90318 CC22TP_RS07530 WP_029697681.1 1573898..1574395(-) (comGF) [Staphylococcus aureus strain TPS6281]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAGAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (69-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	97.576	100	0.976
comGF	Staphylococcus aureus MW2	97.576	100	0.976