Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SGJ46_RS13045 Genome accession   NZ_CP138461
Coordinates   2704546..2705406 (+) Length   286 a.a.
NCBI ID   WP_177155275.1    Uniprot ID   -
Organism   Acinetobacter indicus strain CZH-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2699546..2710406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGJ46_RS13025 secG 2700159..2700488 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  SGJ46_RS13030 tpiA 2700502..2701293 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  SGJ46_RS13035 pilB 2701583..2703289 (+) 1707 WP_016659655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SGJ46_RS13040 pilC 2703320..2704546 (+) 1227 WP_177155276.1 type II secretion system F family protein Machinery gene
  SGJ46_RS13045 pilD 2704546..2705406 (+) 861 WP_177155275.1 prepilin peptidase Machinery gene
  SGJ46_RS13050 coaE 2705417..2706025 (+) 609 WP_177155274.1 dephospho-CoA kinase -
  SGJ46_RS13055 - 2706022..2706942 (-) 921 WP_168396667.1 DMT family transporter -
  SGJ46_RS13060 rlmB 2706960..2707712 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  SGJ46_RS13065 - 2707818..2708141 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32040.28 Da        Isoelectric Point: 7.8299

>NTDB_id=902939 SGJ46_RS13045 WP_177155275.1 2704546..2705406(+) (pilD) [Acinetobacter indicus strain CZH-5]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQASMDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=902939 SGJ46_RS13045 WP_177155275.1 2704546..2705406(+) (pilD) [Acinetobacter indicus strain CZH-5]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCAGCATGGATGAAAGCAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

79.298

99.65

0.79
  pilD Acinetobacter baumannii D1279779

78.947

99.65

0.787
  pilD Vibrio cholerae strain A1552

54.511

93.007

0.507
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437