NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	SBO70_RS13840	Genome accession	NZ_CP137890
Coordinates	2805659..2806273 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain GJJK74
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2800659..2811273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SBO70_RS13810 (SBO70_13810)	rpsT	2800962..2801228 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
SBO70_RS13815 (SBO70_13815)	holA	2801245..2802286 (-)	1042	Protein_2682	DNA polymerase III subunit delta	-
SBO70_RS13820 (SBO70_13820)	-	2802326..2802477 (-)	152	Protein_2683	hypothetical protein	-
SBO70_RS13825 (SBO70_13825)	-	2802518..2802652 (+)	135	WP_003152870.1	YqzM family protein	-
SBO70_RS13830 (SBO70_13830)	comEC	2802671..2805022 (-)	2352	WP_062623529.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SBO70_RS13835 (SBO70_13835)	-	2805023..2805592 (-)	570	WP_003152868.1	ComE operon protein 2	-
SBO70_RS13840 (SBO70_13840)	comEA	2805659..2806273 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
SBO70_RS13845 (SBO70_13845)	comER	2806332..2807153 (+)	822	WP_039254409.1	late competence protein ComER	-
SBO70_RS13850 (SBO70_13850)	-	2807222..2807959 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
SBO70_RS13855 (SBO70_13855)	rsfS	2807956..2808312 (-)	357	WP_007408260.1	ribosome silencing factor	-
SBO70_RS13860 (SBO70_13860)	yqeK	2808330..2808890 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SBO70_RS13865 (SBO70_13865)	-	2808880..2809449 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
SBO70_RS13870 (SBO70_13870)	yhbY	2809460..2809750 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
SBO70_RS13875 (SBO70_13875)	aroE	2809744..2810586 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=901192 SBO70_RS13840 WP_015240245.1 2805659..2806273(-) (comEA) [Bacillus velezensis strain GJJK74]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=901192 SBO70_RS13840 WP_015240245.1 2805659..2806273(-) (comEA) [Bacillus velezensis strain GJJK74]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0901192