Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SBK94_RS12300 Genome accession   NZ_CP137889
Coordinates   2517845..2518915 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain N23     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512845..2523915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBK94_RS12250 (SBK94_12250) sipW 2512905..2513489 (-) 585 WP_014305408.1 signal peptidase I SipW -
  SBK94_RS12255 (SBK94_12255) tapA 2513461..2514132 (-) 672 WP_014305409.1 amyloid fiber anchoring/assembly protein TapA -
  SBK94_RS12260 (SBK94_12260) - 2514391..2514720 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  SBK94_RS12265 (SBK94_12265) - 2514760..2514939 (-) 180 WP_003153093.1 YqzE family protein -
  SBK94_RS12270 (SBK94_12270) comGG 2514996..2515373 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  SBK94_RS12275 (SBK94_12275) comGF 2515374..2515874 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  SBK94_RS12280 (SBK94_12280) comGE 2515783..2516097 (-) 315 WP_041481885.1 competence type IV pilus minor pilin ComGE Machinery gene
  SBK94_RS12285 (SBK94_12285) comGD 2516081..2516518 (-) 438 WP_014305413.1 competence type IV pilus minor pilin ComGD Machinery gene
  SBK94_RS12290 (SBK94_12290) comGC 2516508..2516816 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  SBK94_RS12295 (SBK94_12295) comGB 2516821..2517858 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  SBK94_RS12300 (SBK94_12300) comGA 2517845..2518915 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  SBK94_RS12305 (SBK94_12305) - 2519107..2520057 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  SBK94_RS12310 (SBK94_12310) - 2520203..2521504 (+) 1302 WP_014305416.1 hemolysin family protein -
  SBK94_RS12315 (SBK94_12315) - 2521542..2522372 (-) 831 WP_003153080.1 STAS domain-containing protein -
  SBK94_RS12325 (SBK94_12325) - 2522578..2522958 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  SBK94_RS12330 (SBK94_12330) - 2523195..2523440 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=901114 SBK94_RS12300 WP_003153083.1 2517845..2518915(-) (comGA) [Bacillus velezensis strain N23]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=901114 SBK94_RS12300 WP_003153083.1 2517845..2518915(-) (comGA) [Bacillus velezensis strain N23]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374