Detailed information NTG0899418

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: R6U80_RS11895
• Genome accession: NZ_CP137623
• Coordinates: 2386092..2386538 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis strain NCK401
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2381092..2391538

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6U80_RS11860 (R6U80_11860)	-	2381209..2381586 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
R6U80_RS11865 (R6U80_11865)	-	2381791..2382657 (+)	867	WP_025017140.1	RluA family pseudouridine synthase	-
R6U80_RS11870 (R6U80_11870)	-	2382695..2383504 (-)	810	WP_014570791.1	metal ABC transporter permease	-
R6U80_RS11875 (R6U80_11875)	-	2383497..2384234 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
R6U80_RS11880 (R6U80_11880)	-	2384411..2385253 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
R6U80_RS11885 (R6U80_11885)	-	2385250..2385687 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
R6U80_RS11890 (R6U80_11890)	comGG	2385769..2386053 (-)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
R6U80_RS11895 (R6U80_11895)	comGF	2386092..2386538 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
R6U80_RS11900 (R6U80_11900)	comGE	2386501..2386797 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
R6U80_RS11905 (R6U80_11905)	comGD	2386769..2387167 (-)	399	WP_230315852.1	competence type IV pilus minor pilin ComGD	Machinery gene
R6U80_RS11910 (R6U80_11910)	comGC	2387160..2387543 (-)	384	WP_313791889.1	competence type IV pilus major pilin ComGC	Machinery gene
R6U80_RS11915 (R6U80_11915)	comGB	2387557..2388630 (-)	1074	WP_081041295.1	competence type IV pilus assembly protein ComGB	Machinery gene
R6U80_RS11920 (R6U80_11920)	comGA	2388524..2389462 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=899418 R6U80_RS11895 WP_029344525.1 2386092..2386538(-) (comGF) [Lactococcus lactis strain NCK401]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=899418 R6U80_RS11895 WP_029344525.1 2386092..2386538(-) (comGF) [Lactococcus lactis strain NCK401]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385