Detailed information NTG0089646

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: LDN16_RS03450
• Genome accession: NZ_AP024961
• Coordinates: 727990..728439 (-)
• Length: 149 a.a.
• NCBI ID: WP_010946364.1
• Uniprot ID: Q5ZXV4
• Organism: Legionella pneumophila strain 80-045
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 722990..733439

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDN16_RS03430 (LEG80045_06520)	-	723264..723656 (-)	393	WP_011214843.1	hypothetical protein	-
LDN16_RS03435 (LEG80045_06530)	-	723793..724170 (+)	378	WP_038837636.1	hypothetical protein	-
LDN16_RS03440 (LEG80045_06540)	-	724372..725679 (+)	1308	WP_161514101.1	tetratricopeptide repeat protein	-
LDN16_RS03445 (LEG80045_06550)	comEC	725705..727912 (-)	2208	WP_038837641.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LDN16_RS03450 (LEG80045_06560)	pilE	727990..728439 (-)	450	WP_010946364.1	type IV pilin protein	Machinery gene
LDN16_RS03455 (LEG80045_06570)	-	728451..731960 (-)	3510	WP_161504147.1	pilus assembly protein	-
LDN16_RS03460 (LEG80045_06580)	-	731973..732485 (-)	513	WP_027219362.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16296.57 Da
• Isoelectric Point: 7.6922

>NTDB_id=89646 LDN16_RS03450 WP_010946364.1 727990..728439(-) (pilE) [Legionella pneumophila strain 80-045]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=89646 LDN16_RS03450 WP_010946364.1 727990..728439(-) (pilE) [Legionella pneumophila strain 80-045]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCGGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAATCCCGTCGTGCCGATGCTC
ACGCCACATTGACACAAGATCAAATTATTTTAGAACGCTGCTACTCACAGAATTTTTCTTATGCTGCGGCGTGTGGCGCC
TTACCAGCATTTCCGCAAACAACTCCGAACGGATACTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACTGCAACCCCTGTTGGAACTCAAGCCAAAGATACCGAGTGCGCCAGCATGTCAATTAACCAGGCCAATGTAAAAACAG
CAGTAGATTCCTCTGCCAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: Q5ZXV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	100	100	1
pilE	Legionella pneumophila strain ERS1305867	100	100	1