Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   R4700_RS05395 Genome accession   NZ_CP137112
Coordinates   1046541..1047839 (-) Length   432 a.a.
NCBI ID   WP_050095242.1    Uniprot ID   A0A4J2AYZ8
Organism   Streptococcus pneumoniae strain 02H2025     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041541..1052839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4700_RS05375 rplI 1042696..1043148 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  R4700_RS05380 - 1043145..1045118 (-) 1974 WP_044790958.1 DHH family phosphoesterase -
  R4700_RS05385 hpf 1045254..1045802 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R4700_RS05390 comFC/cflB 1045882..1046544 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  R4700_RS05395 comFA/cflA 1046541..1047839 (-) 1299 WP_050095242.1 DEAD/DEAH box helicase Machinery gene
  R4700_RS05400 - 1047895..1048530 (+) 636 WP_000395729.1 YigZ family protein -
  R4700_RS05405 cysK 1048814..1049734 (+) 921 WP_000029866.1 cysteine synthase A -
  R4700_RS05410 - 1049973..1050323 (-) 351 Protein_1027 IS66 family transposase -
  R4700_RS05415 - 1050323..1050615 (+) 293 Protein_1028 transposase -
  R4700_RS05420 tnpB 1050756..1051079 (-) 324 WP_000973416.1 IS66 family insertion sequence element accessory protein TnpB -
  R4700_RS05425 tsf 1051325..1052365 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49590.61 Da        Isoelectric Point: 8.7540

>NTDB_id=896038 R4700_RS05395 WP_050095242.1 1046541..1047839(-) (comFA/cflA) [Streptococcus pneumoniae strain 02H2025]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKRKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=896038 R4700_RS05395 WP_050095242.1 1046541..1047839(-) (comFA/cflA) [Streptococcus pneumoniae strain 02H2025]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGATAAGCGAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4J2AYZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387