Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   LDO07_RS06610 Genome accession   NZ_AP024937
Coordinates   1223026..1223727 (+) Length   233 a.a.
NCBI ID   WP_223965553.1    Uniprot ID   A0AAD1NXB7
Organism   Thermus thermophilus strain AK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1218026..1228727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO07_RS06585 (TthAK1_12930) ilvB 1218462..1220150 (-) 1689 WP_223965550.1 biosynthetic-type acetolactate synthase large subunit -
  LDO07_RS06590 (TthAK1_12940) - 1220306..1221286 (-) 981 WP_018461695.1 YpdA family putative bacillithiol disulfide reductase -
  LDO07_RS06595 (TthAK1_12950) pilA/pilA1 1221452..1221922 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  LDO07_RS06600 (TthAK1_12960) - 1221978..1222451 (+) 474 WP_223965551.1 GspH/FimT family protein -
  LDO07_RS06605 (TthAK1_12970) pilA/pilA2 1222469..1223029 (+) 561 WP_267461250.1 type II secretion system protein Machinery gene
  LDO07_RS06610 (TthAK1_12980) pilA/pilA3 1223026..1223727 (+) 702 WP_223965553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LDO07_RS06615 (TthAK1_12990) comZ 1223738..1225414 (+) 1677 WP_223965554.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  LDO07_RS06620 (TthAK1_13000) - 1225635..1225985 (+) 351 WP_143585831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LDO07_RS06625 (TthAK1_13010) - 1226234..1226614 (+) 381 WP_223965555.1 DUF1640 domain-containing protein -
  LDO07_RS06630 (TthAK1_13020) - 1226690..1227232 (+) 543 WP_223966753.1 Uma2 family endonuclease -
  LDO07_RS06635 (TthAK1_13030) - 1227222..1227434 (+) 213 WP_223965556.1 DUF2281 domain-containing protein -
  LDO07_RS06640 (TthAK1_13040) - 1227533..1228090 (-) 558 WP_223965557.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24905.32 Da        Isoelectric Point: 7.4615

>NTDB_id=89560 LDO07_RS06610 WP_223965553.1 1223026..1223727(+) (pilA/pilA3) [Thermus thermophilus strain AK1]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGDQNQAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVSATPTSGSDCTTPSPNYQPLAEGILALDIQYQCS
DGTTRNTPDCGANAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=89560 LDO07_RS06610 WP_223965553.1 1223026..1223727(+) (pilA/pilA3) [Thermus thermophilus strain AK1]
ATGAAGCGCGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAACTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGTGGCGACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAAGGGGACACCTTGCGTCGGAGCGACGTGAGCGCCACACCCACCTCGGGGA
GCGACTGCACCACCCCTTCCCCCAACTACCAACCCCTGGCCGAGGGCATCCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGCACCACCCGAAACACTCCGGACTGCGGCGCAAACGCCTACCCCCGCTCGGCCAAGGTTACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

93.562

100

0.936

Multiple sequence alignment