Detailed information NTG0895504

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAHT64_RS07650
• Genome accession: NZ_CP155054
• Coordinates: 1466864..1467481 (+)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: P39694
• Organism: Bacillus subtilis subsp. subtilis strain R38
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1461864..1472481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHT64_RS07615 (AAHT64_07615)	aroE	1462522..1463364 (+)	843	WP_003229967.1	shikimate dehydrogenase	-
AAHT64_RS07620 (AAHT64_07620)	yhbY	1463358..1463648 (+)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AAHT64_RS07625 (AAHT64_07625)	nadD	1463660..1464229 (+)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AAHT64_RS07630 (AAHT64_07630)	yqeK	1464219..1464779 (+)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAHT64_RS07635 (AAHT64_07635)	rsfS	1464797..1465153 (+)	357	WP_003229971.1	ribosome silencing factor	-
AAHT64_RS07640 (AAHT64_07640)	yqeM	1465150..1465893 (+)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
AAHT64_RS07645 (AAHT64_07645)	comER	1465959..1466780 (-)	822	WP_004398597.1	late competence protein ComER	-
AAHT64_RS07650 (AAHT64_07650)	comEA	1466864..1467481 (+)	618	WP_004398514.1	competence protein ComEA	Machinery gene
AAHT64_RS07655 (AAHT64_07655)	comEB	1467548..1468117 (+)	570	WP_003229978.1	ComE operon protein 2	-
AAHT64_RS07660 (AAHT64_07660)	comEC	1468121..1470451 (+)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAHT64_RS07665 (AAHT64_07665)	yqzM	1470491..1470625 (-)	135	WP_003229983.1	YqzM family protein	-
AAHT64_RS07670 (AAHT64_07670)	-	1470666..1470815 (+)	150	WP_003229985.1	hypothetical protein	-
AAHT64_RS07675 (AAHT64_07675)	holA	1470855..1471898 (+)	1044	WP_345835657.1	DNA polymerase III subunit delta	-
AAHT64_RS07680 (AAHT64_07680)	rpsT	1471913..1472179 (-)	267	WP_003229989.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=895504 AAHT64_RS07650 WP_004398514.1 1466864..1467481(+) (comEA) [Bacillus subtilis subsp. subtilis strain R38]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=895504 AAHT64_RS07650 WP_004398514.1 1466864..1467481(+) (comEA) [Bacillus subtilis subsp. subtilis strain R38]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

3D structure

• PDB: 8DFK

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39