Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   R4535_RS11700 Genome accession   NZ_CP137091
Coordinates   2591104..2591694 (-) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586104..2596694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS11680 (R4535_11655) aroB 2587031..2588116 (-) 1086 WP_001960111.1 3-dehydroquinate synthase -
  R4535_RS11685 (R4535_11660) aroK 2588144..2588668 (-) 525 WP_000818616.1 shikimate kinase AroK -
  R4535_RS11690 (R4535_11665) pilQ 2588855..2590570 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  R4535_RS11695 (R4535_11670) pilP 2590596..2591114 (-) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  R4535_RS11700 (R4535_11675) pilO 2591104..2591694 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  R4535_RS11705 (R4535_11680) pilN 2591687..2592271 (-) 585 WP_175597460.1 PilN domain-containing protein Machinery gene
  R4535_RS11710 (R4535_11685) pilM 2592261..2593277 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  R4535_RS11715 (R4535_11690) - 2593420..2595897 (+) 2478 WP_057557137.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=894971 R4535_RS11700 WP_000157690.1 2591104..2591694(-) (pilO) [Vibrio cholerae strain DL4211]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=894971 R4535_RS11700 WP_000157690.1 2591104..2591694(-) (pilO) [Vibrio cholerae strain DL4211]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCCCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694