Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R4535_RS11710 Genome accession   NZ_CP137091
Coordinates   2592261..2593277 (-) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2587261..2598277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS11685 (R4535_11660) aroK 2588144..2588668 (-) 525 WP_000818616.1 shikimate kinase AroK -
  R4535_RS11690 (R4535_11665) pilQ 2588855..2590570 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  R4535_RS11695 (R4535_11670) pilP 2590596..2591114 (-) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  R4535_RS11700 (R4535_11675) pilO 2591104..2591694 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  R4535_RS11705 (R4535_11680) pilN 2591687..2592271 (-) 585 WP_175597460.1 PilN domain-containing protein Machinery gene
  R4535_RS11710 (R4535_11685) pilM 2592261..2593277 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  R4535_RS11715 (R4535_11690) - 2593420..2595897 (+) 2478 WP_057557137.1 penicillin-binding protein 1A -
  R4535_RS11720 (R4535_11695) oxyR 2595972..2596865 (-) 894 WP_033932952.1 DNA-binding transcriptional regulator OxyR -
  R4535_RS11725 (R4535_11700) - 2597005..2597736 (+) 732 WP_000495223.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=894973 R4535_RS11710 WP_000520444.1 2592261..2593277(-) (pilM) [Vibrio cholerae strain DL4211]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=894973 R4535_RS11710 WP_000520444.1 2592261..2593277(-) (pilM) [Vibrio cholerae strain DL4211]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGTTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485