Detailed information NTG0894746

Bioinformatically predicted

Overview

• Name: recG
• Type: Machinery gene
• Locus tag: AAVC70_RS19290
• Genome accession: NZ_CP154898
• Coordinates: 3512754..3514802 (+)
• Length: 682 a.a.
• NCBI ID: WP_013352228.1
• Uniprot ID: A0A9P1JH06
• Organism: Bacillus amyloliquefaciens strain Fad 97
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3507754..3519802

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVC70_RS19260 (AAVC70_19260)	spoVM	3508398..3508478 (+)	81	WP_003154329.1	stage V sporulation protein SpoVM	-
AAVC70_RS19265 (AAVC70_19265)	rpmB	3508555..3508743 (-)	189	WP_003154328.1	50S ribosomal protein L28	-
AAVC70_RS19270 (AAVC70_19270)	-	3509003..3509365 (+)	363	WP_013352224.1	Asp23/Gls24 family envelope stress response protein	-
AAVC70_RS19275 (AAVC70_19275)	-	3509381..3511048 (+)	1668	WP_013352225.1	DAK2 domain-containing protein	-
AAVC70_RS19280 (AAVC70_19280)	sdaAB	3511191..3511853 (+)	663	WP_013352226.1	L-serine ammonia-lyase, iron-sulfur-dependent subunit beta	-
AAVC70_RS19285 (AAVC70_19285)	sdaAA	3511874..3512776 (+)	903	WP_013352227.1	L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha	-
AAVC70_RS19290 (AAVC70_19290)	recG	3512754..3514802 (+)	2049	WP_013352228.1	ATP-dependent DNA helicase RecG	Machinery gene
AAVC70_RS19295 (AAVC70_19295)	fapR	3514909..3515475 (+)	567	WP_013352229.1	transcription factor FapR	-
AAVC70_RS19300 (AAVC70_19300)	plsX	3515489..3516487 (+)	999	WP_013352230.1	phosphate acyltransferase PlsX	-
AAVC70_RS19305 (AAVC70_19305)	fabD	3516506..3517459 (+)	954	WP_013352231.1	ACP S-malonyltransferase	-
AAVC70_RS19310 (AAVC70_19310)	fabG	3517452..3518192 (+)	741	WP_013352232.1	3-oxoacyl-[acyl-carrier-protein] reductase	-
AAVC70_RS19315 (AAVC70_19315)	acpP	3518277..3518510 (+)	234	WP_003154310.1	acyl carrier protein	-
AAVC70_RS19320 (AAVC70_19320)	rncS	3518649..3519398 (+)	750	WP_013352233.1	ribonuclease III	-

Sequence

Protein

• Length: 682 a.a.
• Molecular weight: 77907.35 Da
• Isoelectric Point: 6.9229

>NTDB_id=894746 AAVC70_RS19290 WP_013352228.1 3512754..3514802(+) (recG) [Bacillus amyloliquefaciens strain Fad 97]
MKHHQQTSIAEIKGIGPETEKTLQELGIYDISDLLNYFPYRYDDYELRDLEEVKHEERVTVEGKVHSEPSLTYYGKKRNR
LTFRVLVGNYLITAVCFNRPYLKKKLTLGSVVTISGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
KEALKHHLDNIADPLPEKLRISYKLPDYKQALQTMHQPETRESLQQARRRFVYEEFLLFQLKMQAFRKAEREQSKGISHV
FPAEKLAAFTASLPFSLTNAQMRVLREITADLTSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFANEDVNIALLTSSVKGKRRRELLERLALGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVDQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKQIETYWVKHDMLERILAFIEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSEVYRGKWNVGLMHGKLHSDEKDQVMREFSANQCQVLVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGDHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVSSEAFWKDDEYRMLRAQLLSSGVLEGEKLS

Nucleotide

• Length: 2049 bp

>NTDB_id=894746 AAVC70_RS19290 WP_013352228.1 3512754..3514802(+) (recG) [Bacillus amyloliquefaciens strain Fad 97]
GTGAAACACCATCAGCAAACTAGTATAGCGGAGATTAAGGGCATTGGGCCGGAAACAGAAAAAACATTGCAAGAATTGGG
TATCTATGACATTTCTGATCTTCTGAATTATTTCCCTTACCGTTATGATGACTATGAGCTGAGGGATTTGGAAGAAGTTA
AACATGAAGAAAGAGTTACAGTAGAAGGGAAGGTTCATTCAGAACCTTCTCTTACCTATTACGGCAAAAAACGAAACAGG
CTGACATTCAGGGTGCTTGTCGGCAATTATTTAATTACCGCGGTCTGCTTTAACCGTCCGTACTTAAAAAAGAAACTGAC
GCTCGGTTCTGTCGTCACGATATCCGGGAAATGGGATAAACACAGGCAGACCATTTCTGTGCAGGAATTAAAAAACGGGC
CTCATCAAGAAGATAAAAGCATTGAGCCTGTTTATTCCGTTAAAGAAAACGTCACCGTTAAAATGATGAGGCGGTTTATT
AAGGAAGCTTTGAAGCACCATTTGGACAATATTGCCGATCCGCTTCCTGAAAAATTGAGAATCAGTTACAAGCTGCCTGA
TTACAAACAAGCCCTGCAGACGATGCACCAGCCGGAAACGAGGGAATCGTTACAGCAGGCAAGACGCAGGTTTGTTTACG
AAGAATTCTTATTATTTCAGCTGAAAATGCAGGCGTTCCGTAAAGCGGAAAGAGAACAGTCAAAAGGCATCAGCCATGTG
TTTCCGGCTGAAAAGCTCGCCGCTTTTACAGCCAGCCTTCCGTTTTCGCTCACGAACGCACAGATGCGCGTGCTCCGTGA
AATTACCGCTGATTTGACATCTCCTTACCGTATGAACCGTCTGCTGCAAGGCGATGTCGGGTCAGGGAAAACGGCCGTCG
CCGCCATCGCTTTGTACGCTGCGATCCTGTCGGGGTATCAGGGGGCTTTAATGGTGCCGACTGAAATTCTGGCCGAACAG
CATGCCGACTCTCTCGTATCGTTATTTGCAAATGAAGATGTGAACATCGCGCTTTTGACGAGTTCTGTTAAAGGAAAGCG
GCGCAGAGAGCTTTTGGAGCGGCTTGCTCTCGGAGAAATTGATATTTTAGTAGGGACCCATGCTTTAATCCAGGATGAAG
TGGAATTTAAAGCGCTGAGCCTTGTCATTACGGACGAGCAGCATCGGTTCGGGGTCGACCAGCGCAAAAAACTCAGGAAT
AAAGGCCAGGACCCGGATGTGCTGTTTATGACAGCCACCCCGATACCGAGAACATTAGCCATTACCGTCTTCGGAGAAAT
GGATGTTTCCGTTATAGATGAAATGCCCGCGGGACGAAAACAAATCGAAACGTATTGGGTGAAACACGACATGCTGGAGC
GGATTTTGGCTTTTATTGAAAAAGAGCTGAAGCAGGGAAGGCAGGCCTATATTATCTGCCCGCTCATTGAAGAGTCGGAT
AAGCTGGATGTTCAGAATGCGATTGACGTATATAACATGCTGTCTGAGGTTTACCGCGGCAAATGGAATGTCGGTCTGAT
GCACGGGAAGCTCCATTCTGATGAAAAAGATCAGGTGATGAGAGAATTCAGCGCCAATCAATGTCAGGTTCTTGTGTCAA
CAACTGTCGTAGAAGTCGGGGTGAACGTGCCGAATGCGACGATTATGGTCATCTATGACGCCGACCGTTTCGGGCTGTCT
CAGCTCCATCAGCTCCGCGGCCGGGTCGGACGGGGAGACCATCAGTCTTTCTGCATCTTGATGGCCGATCCGAAATCCGA
AACCGGGAAAGAACGGATGAGAATCATGTCGGAGACAAACGACGGCTTTGAACTGTCTGAAAAGGACCTTGAGCTGCGCG
GTCCCGGCGATTTCTTCGGGAAAAAACAGAGCGGTATGCCCGAGTTCAAGGTGGCGGATATGGTTCATGACTACAGAGCT
TTGGAAACGGCCCGCCAGGATGCGGCCAATCTGGTGTCATCAGAGGCTTTTTGGAAAGATGACGAGTACCGCATGCTCCG
CGCTCAACTGCTCTCAAGCGGTGTCTTAGAAGGGGAGAAATTAAGCTGA

Domains

Predicted by InterProScan.

• RecG wedge domain (RecG_wedge) (11-164)

• DEAD/DEAH box helicase (DEAD) (277-415)

• ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C) (599-670)

• Helicase conserved C-terminal domain (Helicase_C) (452-572)

3D structure

• AlphaFold DB: A0A9P1JH06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recG	Bacillus subtilis subsp. subtilis str. 168	90.029	100	0.9
recG/mmsA	Streptococcus pneumoniae R6	49.776	98.387	0.49
recG/mmsA	Streptococcus pneumoniae R36A	49.776	98.387	0.49
recG	Neisseria meningitidis strain C311	38.897	95.748	0.372