Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   AAVB98_RS00720 Genome accession   NZ_CP154895
Coordinates   139552..141495 (-) Length   647 a.a.
NCBI ID   WP_013352220.1    Uniprot ID   A0A9P1NHU6
Organism   Bacillus amyloliquefaciens strain Fad 77     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 134552..146495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB98_RS00690 (AAVB98_00690) - 136226..136588 (-) 363 WP_013352224.1 Asp23/Gls24 family envelope stress response protein -
  AAVB98_RS00695 (AAVB98_00695) rpmB 136848..137036 (+) 189 WP_003154328.1 50S ribosomal protein L28 -
  AAVB98_RS00700 (AAVB98_00700) spoVM 137113..137193 (-) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  AAVB98_RS00705 (AAVB98_00705) - 137280..137924 (-) 645 WP_013352223.1 thiamine diphosphokinase -
  AAVB98_RS00710 (AAVB98_00710) rpe 137992..138645 (-) 654 WP_013352222.1 ribulose-phosphate 3-epimerase -
  AAVB98_RS00715 (AAVB98_00715) rsgA 138647..139537 (-) 891 WP_013352221.1 ribosome small subunit-dependent GTPase A -
  AAVB98_RS00720 (AAVB98_00720) stkP 139552..141495 (-) 1944 WP_013352220.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  AAVB98_RS00725 (AAVB98_00725) - 141489..142250 (-) 762 WP_013352219.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  AAVB98_RS00730 (AAVB98_00730) rlmN 142256..143347 (-) 1092 WP_013352218.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AAVB98_RS00735 (AAVB98_00735) rsmB 143351..144694 (-) 1344 WP_061573886.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  AAVB98_RS00740 (AAVB98_00740) fmt 144681..145634 (-) 954 WP_061573885.1 methionyl-tRNA formyltransferase -
  AAVB98_RS00745 (AAVB98_00745) def 145639..146121 (-) 483 WP_061573884.1 peptide deformylase -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71540.31 Da        Isoelectric Point: 5.1147

>NTDB_id=894481 AAVB98_RS00720 WP_013352220.1 139552..141495(-) (stkP) [Bacillus amyloliquefaciens strain Fad 77]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYITAHGPLHPKEALSVMKQIVSAIAHAHHNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKMPFDGESAVSIALKHLQAETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAIAALQAETPAETDDKEQPSA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKEVSVPDVRGMKYEKAEALLEKNGLQADPDMTDIEDEKIEEGLMV
KTDPKAGSAVKEGSSVKLYKSIGKPKTQLIDVKGRPIDDAKKTLKDKGFKHVNVKEENDDSAAGTVIDQNPSAGTDMVVS
DDKVSLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKEAHSDDVPEGQVMKQEPAAGTAVKPGSDVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=894481 AAVB98_RS00720 WP_013352220.1 139552..141495(-) (stkP) [Bacillus amyloliquefaciens strain Fad 77]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCGAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTTGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGACATTTATTATATTGTCATGGAATATGTGGAAGGCATGACGTTAAAGGAATACATAACCGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCTGTTATGAAGCAGATCGTTTCAGCCATCGCCCATGCCCATCATAACCATATCGTGCACCGGG
ATATCAAACCGCACAATATTTTGATAGATCATTTAGGCCATATCAAGGTGACGGATTTCGGCATCGCCACAGCCCTCACT
TCGACGACAATTACACATACGAATTCTGTGCTCGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCGGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGCAAAATGCCTTTTGACGGAGAAT
CAGCCGTCAGCATTGCGTTAAAGCATCTGCAAGCAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTGGAAAACATTATTTTAAAGGCAACCGCCAAAGATCCGTTTCACCGCTATGAAAGCGCAGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTCTCCGTGCAGGATGATGAGGAAATGACGAAAGCGATTC
CCGTTATTACTGACGGTGCGATAGCCGCGCTTCAGGCGGAAACGCCGGCAGAGACGGATGATAAAGAACAGCCGTCCGCC
AAAAAGAAGAAACGGAAATGGCCGTGGGTTTTGCTGGCTGTCTGCTTTATTTTCATAATGGCGGCTGTTCTTGCAGTCAC
CGTGTTCCCGTCTTTGTTTATGCCCAAAGAGGTAAGCGTCCCCGATGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGCCTTCAAGCGGATCCTGATATGACGGACATTGAAGATGAAAAAATCGAAGAGGGATTAATGGTC
AAAACTGATCCGAAAGCGGGTTCTGCCGTTAAAGAAGGCTCTTCTGTAAAGCTGTACAAAAGCATCGGGAAACCGAAAAC
CCAGCTGATTGACGTGAAAGGCCGGCCGATCGATGATGCGAAAAAAACGCTTAAGGATAAAGGCTTCAAACATGTCAATG
TAAAAGAAGAGAACGATGACAGTGCGGCTGGCACCGTAATTGATCAGAACCCATCAGCCGGAACCGACATGGTCGTAAGC
GATGATAAAGTCAGCTTAACAGTCAGCCTCGGACCGGCGGACGTTACGCTGAGAGACTTAAAAACATACAGTAAAGAAGC
GGCTTCCGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAGGAAGCTCACTCAGATGACGTGCCTGAGGGACAGG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTGATGTTGAAGTAACGTTTTCGCTCGGGCCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATCCCCTACGAACCCGAACATGAAGGGGACGAGCTCGAAGTGCA
AATCGCCATCGATGATAAAGACCACAGCATCTCTGATACGTATGACTCGTTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1NHU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366