NTDB

Overview

Name	recG	Type	Machinery gene
Locus tag	AAVB98_RS00670	Genome accession	NZ_CP154895
Coordinates	130789..132837 (-)	Length	682 a.a.
NCBI ID	WP_013352228.1	Uniprot ID	A0A9P1JH06
Organism	Bacillus amyloliquefaciens strain Fad 77
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 125789..137837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVB98_RS00640 (AAVB98_00640)	rncS	126193..126942 (-)	750	WP_013352233.1	ribonuclease III	-
AAVB98_RS00645 (AAVB98_00645)	acpP	127081..127314 (-)	234	WP_003154310.1	acyl carrier protein	-
AAVB98_RS00650 (AAVB98_00650)	fabG	127399..128139 (-)	741	WP_013352232.1	3-oxoacyl-[acyl-carrier-protein] reductase	-
AAVB98_RS00655 (AAVB98_00655)	fabD	128132..129085 (-)	954	WP_013352231.1	ACP S-malonyltransferase	-
AAVB98_RS00660 (AAVB98_00660)	plsX	129104..130102 (-)	999	WP_013352230.1	phosphate acyltransferase PlsX	-
AAVB98_RS00665 (AAVB98_00665)	fapR	130116..130682 (-)	567	WP_013352229.1	transcription factor FapR	-
AAVB98_RS00670 (AAVB98_00670)	recG	130789..132837 (-)	2049	WP_013352228.1	ATP-dependent DNA helicase RecG	Machinery gene
AAVB98_RS00675 (AAVB98_00675)	sdaAA	132815..133717 (-)	903	WP_013352227.1	L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha	-
AAVB98_RS00680 (AAVB98_00680)	sdaAB	133738..134400 (-)	663	WP_013352226.1	L-serine ammonia-lyase, iron-sulfur-dependent subunit beta	-
AAVB98_RS00685 (AAVB98_00685)	-	134543..136210 (-)	1668	WP_013352225.1	DAK2 domain-containing protein	-
AAVB98_RS00690 (AAVB98_00690)	-	136226..136588 (-)	363	WP_013352224.1	Asp23/Gls24 family envelope stress response protein	-
AAVB98_RS00695 (AAVB98_00695)	rpmB	136848..137036 (+)	189	WP_003154328.1	50S ribosomal protein L28	-
AAVB98_RS00700 (AAVB98_00700)	spoVM	137113..137193 (-)	81	WP_003154329.1	stage V sporulation protein SpoVM	-

Sequence

Protein

Download Length: 682 a.a. Molecular weight: 77907.35 Da Isoelectric Point: 6.9229

                                >NTDB_id=894480 AAVB98_RS00670 WP_013352228.1 130789..132837(-) (recG) [Bacillus amyloliquefaciens strain Fad 77]

MKHHQQTSIAEIKGIGPETEKTLQELGIYDISDLLNYFPYRYDDYELRDLEEVKHEERVTVEGKVHSEPSLTYYGKKRNR

LTFRVLVGNYLITAVCFNRPYLKKKLTLGSVVTISGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI

KEALKHHLDNIADPLPEKLRISYKLPDYKQALQTMHQPETRESLQQARRRFVYEEFLLFQLKMQAFRKAEREQSKGISHV

FPAEKLAAFTASLPFSLTNAQMRVLREITADLTSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ

HADSLVSLFANEDVNIALLTSSVKGKRRRELLERLALGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVDQRKKLRN

KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKQIETYWVKHDMLERILAFIEKELKQGRQAYIICPLIEESD

KLDVQNAIDVYNMLSEVYRGKWNVGLMHGKLHSDEKDQVMREFSANQCQVLVSTTVVEVGVNVPNATIMVIYDADRFGLS

QLHQLRGRVGRGDHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA

LETARQDAANLVSSEAFWKDDEYRMLRAQLLSSGVLEGEKLS

Nucleotide

Download Length: 2049 bp

                                >NTDB_id=894480 AAVB98_RS00670 WP_013352228.1 130789..132837(-) (recG) [Bacillus amyloliquefaciens strain Fad 77]

GTGAAACACCATCAGCAAACTAGTATAGCGGAGATTAAGGGCATTGGGCCGGAAACAGAAAAAACATTGCAAGAATTGGG

TATCTATGACATTTCTGATCTTCTGAATTATTTCCCTTACCGTTATGATGACTATGAGCTGAGGGATTTGGAAGAAGTTA

AACATGAAGAAAGAGTTACAGTAGAAGGGAAGGTTCATTCAGAACCTTCTCTTACCTATTACGGCAAAAAACGAAACAGG

CTGACATTCAGGGTGCTTGTCGGCAATTATTTAATTACCGCGGTCTGCTTTAACCGTCCGTACTTAAAAAAGAAACTGAC

GCTCGGTTCTGTCGTCACGATATCCGGGAAATGGGATAAACACAGGCAGACCATTTCTGTGCAGGAATTAAAAAACGGGC

CTCATCAAGAAGATAAAAGCATTGAGCCTGTTTATTCCGTTAAAGAAAACGTCACCGTTAAAATGATGAGGCGGTTTATT

AAGGAAGCTTTGAAGCACCATTTGGACAATATTGCCGATCCGCTTCCTGAAAAATTGAGAATCAGTTACAAGCTGCCTGA

TTACAAACAAGCCCTGCAGACGATGCACCAGCCGGAAACGAGGGAATCGTTACAGCAGGCAAGACGCAGGTTTGTTTACG

AAGAATTCTTATTATTTCAGCTGAAAATGCAGGCGTTCCGTAAAGCGGAAAGAGAACAGTCAAAAGGCATCAGCCATGTG

TTTCCGGCTGAAAAGCTCGCCGCTTTTACAGCCAGCCTTCCGTTTTCGCTCACGAACGCACAGATGCGCGTGCTCCGTGA

AATTACCGCTGATTTGACATCTCCTTACCGTATGAACCGTCTGCTGCAAGGCGATGTCGGGTCAGGGAAAACGGCCGTCG

CCGCCATCGCTTTGTACGCTGCGATCCTGTCGGGGTATCAGGGGGCTTTAATGGTGCCGACTGAAATTCTGGCCGAACAG

CATGCCGACTCTCTCGTATCGTTATTTGCAAATGAAGATGTGAACATCGCGCTTTTGACGAGTTCTGTTAAAGGAAAGCG

GCGCAGAGAGCTTTTGGAGCGGCTTGCTCTCGGAGAAATTGATATTTTAGTAGGGACCCATGCTTTAATCCAGGATGAAG

TGGAATTTAAAGCGCTGAGCCTTGTCATTACGGACGAGCAGCATCGGTTCGGGGTCGACCAGCGCAAAAAACTCAGGAAT

AAAGGCCAGGACCCGGATGTGCTGTTTATGACAGCCACCCCGATACCGAGAACATTAGCCATTACCGTCTTCGGAGAAAT

GGATGTTTCCGTTATAGATGAAATGCCCGCGGGACGAAAACAAATCGAAACGTATTGGGTGAAACACGACATGCTGGAGC

GGATTTTGGCTTTTATTGAAAAAGAGCTGAAGCAGGGAAGGCAGGCCTATATTATCTGCCCGCTCATTGAAGAGTCGGAT

AAGCTGGATGTTCAGAATGCGATTGACGTATATAACATGCTGTCTGAGGTTTACCGCGGCAAATGGAATGTCGGTCTGAT

GCACGGGAAGCTCCATTCTGATGAAAAAGATCAGGTGATGAGAGAATTCAGCGCCAATCAATGTCAGGTTCTTGTGTCAA

CAACTGTCGTAGAAGTCGGGGTGAACGTGCCGAATGCGACGATTATGGTCATCTATGACGCCGACCGTTTCGGGCTGTCT

CAGCTCCATCAGCTCCGCGGCCGGGTCGGACGGGGAGACCATCAGTCTTTCTGCATCTTGATGGCCGATCCGAAATCCGA

AACCGGGAAAGAACGGATGAGAATCATGTCGGAGACAAACGACGGCTTTGAACTGTCTGAAAAGGACCTTGAGCTGCGCG

GTCCCGGCGATTTCTTCGGGAAAAAACAGAGCGGTATGCCCGAGTTCAAGGTGGCGGATATGGTTCATGACTACAGAGCT

TTGGAAACGGCCCGCCAGGATGCGGCCAATCTGGTGTCATCAGAGGCTTTTTGGAAAGATGACGAGTACCGCATGCTCCG

CGCTCAACTGCTCTCAAGCGGTGTCTTAGAAGGGGAGAAATTAAGCTGA

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(11-164)

DEAD/DEAH box helicase (DEAD)

(277-415)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A9P1JH06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
recG	Bacillus subtilis subsp. subtilis str. 168	90.029	100	0.9
recG/mmsA	Streptococcus pneumoniae R6	49.776	98.387	0.49
recG/mmsA	Streptococcus pneumoniae R36A	49.776	98.387	0.49
recG	Neisseria meningitidis strain C311	38.897	95.748	0.372

Detailed information NTG0894480