Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAG092_RS13895 Genome accession   NZ_CP154874
Coordinates   2872789..2873415 (+) Length   208 a.a.
NCBI ID   WP_373387129.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2867789..2878415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS13880 (AAG092_13810) - 2868497..2870911 (-) 2415 WP_373387127.1 penicillin-binding protein 1A -
  AAG092_RS13885 (AAG092_13815) pilM 2871096..2872160 (+) 1065 WP_110682985.1 pilus assembly protein PilM Machinery gene
  AAG092_RS13890 (AAG092_13820) - 2872160..2872792 (+) 633 WP_373387128.1 PilN domain-containing protein -
  AAG092_RS13895 (AAG092_13825) pilO 2872789..2873415 (+) 627 WP_373387129.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAG092_RS13900 (AAG092_13830) pilP 2873415..2873938 (+) 524 Protein_2741 type 4a pilus biogenesis lipoprotein PilP -
  AAG092_RS13905 (AAG092_13835) pilQ 2873992..2876052 (+) 2061 WP_373387130.1 type IV pilus secretin PilQ Machinery gene
  AAG092_RS13910 (AAG092_13840) aroK 2876057..2876575 (+) 519 WP_110682990.1 shikimate kinase AroK -
  AAG092_RS13915 (AAG092_13845) aroB 2876625..2877728 (+) 1104 WP_110683039.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 208 a.a.        Molecular weight: 23346.65 Da        Isoelectric Point: 4.3361

>NTDB_id=893904 AAG092_RS13895 WP_373387129.1 2872789..2873415(+) (pilO) [Pseudomonas alcaligenes strain Med1]
MSFSEALESLRKIELSELDVNNLGSWPAAVKFITCILLLVAGLGLGYYFYLQDLQLQLQQQQAQEETLKQEFSNKAAQAA
NLDAYKVQMKEMEESFGMLLRQLPSDTEVPGLLEDITRTGLESGLEFEEIKLQPEVVQQFYIELPIQVSVVGAYHDLATF
VSAVASLPRIVTLHDFEVKQSTGEENSSKLAMSVVAKTYRYNDQGLKK

Nucleotide


Download         Length: 627 bp        

>NTDB_id=893904 AAG092_RS13895 WP_373387129.1 2872789..2873415(+) (pilO) [Pseudomonas alcaligenes strain Med1]
ATGAGTTTCTCAGAAGCGCTTGAGTCATTACGGAAGATTGAGCTTTCCGAGCTTGATGTAAATAACCTCGGCTCCTGGCC
GGCGGCGGTCAAGTTCATCACTTGCATATTGCTGTTGGTGGCTGGGCTGGGGCTGGGGTATTACTTCTATCTGCAGGACC
TGCAGCTGCAATTACAGCAGCAACAGGCGCAGGAAGAGACACTGAAACAGGAGTTCTCCAACAAAGCTGCCCAGGCAGCC
AACCTGGATGCCTACAAGGTCCAGATGAAGGAGATGGAGGAGTCCTTTGGCATGCTGTTGCGGCAGTTGCCCAGTGATAC
CGAGGTTCCGGGCCTGCTCGAAGACATTACGCGCACTGGTCTGGAGAGTGGTCTGGAGTTTGAAGAGATCAAGCTTCAGC
CGGAAGTCGTGCAGCAGTTCTACATAGAGCTGCCTATTCAGGTTTCCGTCGTCGGGGCTTATCACGATCTGGCAACTTTC
GTCAGTGCTGTGGCCAGTTTGCCGCGCATCGTGACCCTGCATGACTTCGAGGTTAAGCAGTCCACGGGTGAAGAAAACTC
TTCCAAACTGGCTATGAGCGTGGTAGCGAAGACCTATCGTTACAATGATCAGGGGCTTAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.939

94.231

0.442
  pilO Acinetobacter baumannii D1279779

45.026

91.827

0.413
  comO/pilO Acinetobacter baylyi ADP1

44.737

91.346

0.409