Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RZV16_RS14710 Genome accession   NZ_CP136578
Coordinates   3426146..3427183 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas cannabis strain DAR82727     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3421146..3432183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV16_RS14685 (RZV16_14635) - 3421531..3422097 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  RZV16_RS14690 (RZV16_14640) - 3422403..3422969 (+) 567 WP_228334036.1 DNA-3-methyladenine glycosylase I -
  RZV16_RS14695 (RZV16_14645) - 3423006..3423905 (+) 900 WP_228314138.1 DUF72 domain-containing protein -
  RZV16_RS14700 (RZV16_14650) - 3423918..3424592 (-) 675 WP_039566430.1 YitT family protein -
  RZV16_RS14705 (RZV16_14655) pilU 3424902..3426032 (-) 1131 WP_039404792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV16_RS14710 (RZV16_14660) pilT 3426146..3427183 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  RZV16_RS14715 (RZV16_14665) - 3427511..3428203 (+) 693 WP_047693785.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZV16_RS14720 (RZV16_14670) proC 3428247..3429101 (+) 855 WP_228334035.1 pyrroline-5-carboxylate reductase -
  RZV16_RS14725 (RZV16_14675) - 3429658..3430080 (+) 423 WP_047693787.1 HU family DNA-binding protein -
  RZV16_RS14730 (RZV16_14680) - 3430183..3430815 (+) 633 WP_184556994.1 M23 family metallopeptidase -
  RZV16_RS14735 (RZV16_14685) - 3430907..3431209 (+) 303 WP_039404739.1 DUF3861 family protein -
  RZV16_RS14740 (RZV16_14690) - 3431381..3431809 (+) 429 WP_228334034.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=891666 RZV16_RS14710 WP_003489919.1 3426146..3427183(-) (pilT) [Xanthomonas cannabis strain DAR82727]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=891666 RZV16_RS14710 WP_003489919.1 3426146..3427183(-) (pilT) [Xanthomonas cannabis strain DAR82727]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTGGACCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGAACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCCACCGGGT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTGGGCGAATTGCGCGACCTGGAGACCATCCGCCTGG
CGCTCACCGCAGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGCCGTACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACACGCAACCAGGCGCGTGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394