Detailed information NTG0891262

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RYH72_RS15635
• Genome accession: NZ_CP136506
• Coordinates: 3421555..3422724 (-)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas syringae pv. actinidiae strain YXH1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3416555..3427724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RYH72_RS15620 (RYH72_003133)	-	3416950..3418014 (+)	1065	WP_003383303.1	microcin C ABC transporter permease YejB	-
RYH72_RS15625 (RYH72_003134)	-	3418014..3419036 (+)	1023	WP_017682835.1	ABC transporter permease	-
RYH72_RS15630 (RYH72_003135)	-	3419038..3420615 (+)	1578	WP_017699732.1	ABC transporter ATP-binding protein	-
RYH72_RS15635 (RYH72_003136)	pilU	3421555..3422724 (-)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RYH72_RS15640 (RYH72_003137)	-	3422879..3423154 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
RYH72_RS15645 (RYH72_003138)	ltrA	3423706..3425073 (+)	1368	WP_032685472.1	group II intron reverse transcriptase/maturase	-
RYH72_RS15650 (RYH72_003139)	-	3425558..3426112 (-)	555	WP_017684828.1	hypothetical protein	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=891262 RYH72_RS15635 WP_017682837.1 3421555..3422724(-) (pilU) [Pseudomonas syringae pv. actinidiae strain YXH1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=891262 RYH72_RS15635 WP_017682837.1 3421555..3422724(-) (pilU) [Pseudomonas syringae pv. actinidiae strain YXH1]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACCCTCAAGCCCGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCCGGCATTGGTCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGGAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATCAGCGAGGAAGAAGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTTCG
CCTCAAACTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365