Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RW107_RS13030 Genome accession   NZ_CP136257
Coordinates   2552787..2553404 (-) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain A9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2547787..2558404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RW107_RS13000 (RW107_13000) rpsT 2548088..2548354 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  RW107_RS13005 (RW107_13005) holA 2548369..2549412 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  RW107_RS13010 (RW107_13010) - 2549452..2549565 (-) 114 WP_122060479.1 hypothetical protein -
  RW107_RS13015 (RW107_13015) yqzM 2549642..2549776 (+) 135 WP_003229983.1 YqzM family protein -
  RW107_RS13020 (RW107_13020) comEC 2549817..2552147 (-) 2331 WP_128441738.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RW107_RS13025 (RW107_13025) comEB 2552151..2552720 (-) 570 WP_003229978.1 ComE operon protein 2 -
  RW107_RS13030 (RW107_13030) comEA 2552787..2553404 (-) 618 WP_032726193.1 competence protein ComEA Machinery gene
  RW107_RS13035 (RW107_13035) comER 2553488..2554309 (+) 822 WP_032726195.1 late competence protein ComER -
  RW107_RS13040 (RW107_13040) yqeM 2554375..2555118 (-) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  RW107_RS13045 (RW107_13045) rsfS 2555115..2555471 (-) 357 WP_003237112.1 ribosome silencing factor -
  RW107_RS13050 (RW107_13050) yqeK 2555489..2556049 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RW107_RS13055 (RW107_13055) nadD 2556039..2556608 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  RW107_RS13060 (RW107_13060) yhbY 2556620..2556910 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  RW107_RS13065 (RW107_13065) aroE 2556904..2557746 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=889899 RW107_RS13030 WP_032726193.1 2552787..2553404(-) (comEA) [Bacillus subtilis strain A9]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=889899 RW107_RS13030 WP_032726193.1 2552787..2553404(-) (comEA) [Bacillus subtilis strain A9]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376