Detailed information NTG0889653

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWA07_RS04610
• Genome accession: NZ_CP136183
• Coordinates: 956629..957747 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain ABO21-A001
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 951629..962747

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWA07_RS04580 (RWA07_04580)	-	952458..953306 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
RWA07_RS04585 (RWA07_04585)	-	953559..954014 (+)	456	WP_000782976.1	bacteriohemerythrin	-
RWA07_RS04590 (RWA07_04590)	-	954167..955234 (+)	1068	WP_125564930.1	hypothetical protein	-
RWA07_RS04595 (RWA07_04595)	-	955234..955557 (+)	324	WP_000442589.1	RnfH family protein	-
RWA07_RS04600 (RWA07_04600)	-	955612..956010 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWA07_RS04605 (RWA07_04605)	fur	956122..956559 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWA07_RS04610 (RWA07_04610)	pilU	956629..957747 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWA07_RS04615 (RWA07_04615)	pilT	957775..958812 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWA07_RS04620 (RWA07_04620)	-	958939..959631 (+)	693	WP_317857556.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=889653 RWA07_RS04610 WP_000347036.1 956629..957747(-) (pilU) [Acinetobacter baumannii strain ABO21-A001]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889653 RWA07_RS04610 WP_000347036.1 956629..957747(-) (pilU) [Acinetobacter baumannii strain ABO21-A001]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392