Detailed information NTG0889447

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RWT85_RS01460
• Genome accession: NZ_CP136179
• Coordinates: 310486..311544 (+)
• Length: 352 a.a.
• NCBI ID: WP_004644147.1
• Uniprot ID: A0A7U3Y305
• Organism: Acinetobacter baumannii strain ABO21-A022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 305486..316544

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWT85_RS01445 (RWT85_01445)	-	306592..306924 (+)	333	WP_000169602.1	hypothetical protein	-
RWT85_RS01450 (RWT85_01450)	-	306927..307751 (+)	825	WP_002022249.1	putative RNA methyltransferase	-
RWT85_RS01455 (RWT85_01455)	ponA	307769..310324 (-)	2556	WP_000736667.1	penicillin-binding protein PBP1a	-
RWT85_RS01460 (RWT85_01460)	pilM	310486..311544 (+)	1059	WP_004644147.1	pilus assembly protein PilM	Machinery gene
RWT85_RS01465 (RWT85_01465)	pilN	311544..312185 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
RWT85_RS01470 (RWT85_01470)	pilO	312182..312922 (+)	741	WP_000076099.1	type 4a pilus biogenesis protein PilO	Machinery gene
RWT85_RS01475 (RWT85_01475)	pilP	312933..313460 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
RWT85_RS01480 (RWT85_01480)	pilQ	313523..315688 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
RWT85_RS01485 (RWT85_01485)	aroK	315700..316269 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39062.61 Da
• Isoelectric Point: 4.4618

>NTDB_id=889447 RWT85_RS01460 WP_004644147.1 310486..311544(+) (pilM) [Acinetobacter baumannii strain ABO21-A022]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLTNPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=889447 RWT85_RS01460 WP_004644147.1 310486..311544(+) (pilM) [Acinetobacter baumannii strain ABO21-A022]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTA
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7U3Y305

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	99.716	100	0.997
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423