Detailed information NTG0889337

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWU60_RS14090
• Genome accession: NZ_CP136172
• Coordinates: 3037988..3039106 (+)
• Length: 372 a.a.
• NCBI ID: WP_317856850.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain ABO21-A051
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3032988..3044106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWU60_RS14080 (RWU60_14080)	-	3036104..3036796 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWU60_RS14085 (RWU60_14085)	pilT	3036923..3037960 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWU60_RS14090 (RWU60_14090)	pilU	3037988..3039106 (+)	1119	WP_317856850.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWU60_RS14095 (RWU60_14095)	fur	3039176..3039613 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWU60_RS14100 (RWU60_14100)	-	3039725..3040123 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWU60_RS14105 (RWU60_14105)	-	3040178..3040501 (-)	324	WP_000442585.1	RnfH family protein	-
RWU60_RS14110 (RWU60_14110)	-	3040501..3041568 (-)	1068	WP_310665461.1	hypothetical protein	-
RWU60_RS14115 (RWU60_14115)	-	3041721..3042176 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWU60_RS14120 (RWU60_14120)	-	3042428..3043276 (-)	849	WP_317856851.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41935.14 Da
• Isoelectric Point: 6.6944

>NTDB_id=889337 RWU60_RS14090 WP_317856850.1 3037988..3039106(+) (pilU) [Acinetobacter baumannii strain ABO21-A051]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889337 RWU60_RS14090 WP_317856850.1 3037988..3039106(+) (pilU) [Acinetobacter baumannii strain ABO21-A051]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAACTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392