Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RWU60_RS15815 Genome accession   NZ_CP136172
Coordinates   3393709..3394509 (-) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain ABO21-A051     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3388709..3399509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWU60_RS15795 (RWU60_15795) - 3389774..3390472 (-) 699 WP_000058788.1 YfgM family protein -
  RWU60_RS15800 (RWU60_15800) hisS 3390501..3391793 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  RWU60_RS15805 (RWU60_15805) ispG 3391790..3392905 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RWU60_RS15810 (RWU60_15810) - 3392927..3393718 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  RWU60_RS15815 (RWU60_15815) pilF 3393709..3394509 (-) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RWU60_RS15820 (RWU60_15820) rlmN 3394525..3395757 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  RWU60_RS15825 (RWU60_15825) ndk 3395880..3396311 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  RWU60_RS15830 (RWU60_15830) iscX 3396425..3396625 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  RWU60_RS15835 (RWU60_15835) - 3396725..3397276 (-) 552 WP_000769125.1 phosphatase PAP2 family protein -
  RWU60_RS15840 (RWU60_15840) - 3397301..3398599 (-) 1299 WP_086238243.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=889340 RWU60_RS15815 WP_001984465.1 3393709..3394509(-) (pilF) [Acinetobacter baumannii strain ABO21-A051]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=889340 RWU60_RS15815 WP_001984465.1 3393709..3394509(-) (pilF) [Acinetobacter baumannii strain ABO21-A051]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722