Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RS399_RS19570 Genome accession   NZ_CP135973
Coordinates   3869457..3870527 (+) Length   356 a.a.
NCBI ID   WP_003236962.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain SI3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3864457..3875527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS399_RS19535 (RS399_19535) mgsR 3864854..3865231 (+) 378 WP_003236975.1 transcriptional regulator MgsR -
  RS399_RS19545 (RS399_19545) - 3865456..3866295 (+) 840 WP_084993715.1 STAS domain-containing protein -
  RS399_RS19550 (RS399_19550) - 3866352..3867680 (-) 1329 WP_084993716.1 hemolysin family protein -
  RS399_RS19555 (RS399_19555) corA 3867824..3868768 (+) 945 WP_003236968.1 magnesium transporter CorA -
  RS399_RS19560 (RS399_19560) - 3868831..3869241 (+) 411 WP_084993718.1 CBS domain-containing protein -
  RS399_RS19565 (RS399_19565) - 3869264..3869494 (+) 231 WP_315957522.1 hypothetical protein -
  RS399_RS19570 (RS399_19570) comGA 3869457..3870527 (+) 1071 WP_003236962.1 competence protein ComGA Machinery gene
  RS399_RS19575 (RS399_19575) comGB 3870514..3871551 (+) 1038 WP_084993720.1 competence type IV pilus assembly protein ComGB Machinery gene
  RS399_RS19580 (RS399_19580) comGC 3871565..3871861 (+) 297 WP_003236957.1 comG operon protein ComGC Machinery gene
  RS399_RS19585 (RS399_19585) comGD 3871851..3872282 (+) 432 WP_084993722.1 competence type IV pilus minor pilin ComGD Machinery gene
  RS399_RS19590 (RS399_19590) comGE 3872266..3872613 (+) 348 WP_084993724.1 competence type IV pilus minor pilin ComGE Machinery gene
  RS399_RS19595 (RS399_19595) comGF 3872639..3873022 (+) 384 WP_084993726.1 competence type IV pilus minor pilin ComGF Machinery gene
  RS399_RS19600 (RS399_19600) comGG 3873023..3873397 (+) 375 WP_084993728.1 competence type IV pilus minor pilin ComGG Machinery gene
  RS399_RS19605 (RS399_19605) - 3873468..3873647 (+) 180 WP_003236949.1 YqzE family protein -
  RS399_RS19610 (RS399_19610) - 3873689..3874015 (-) 327 WP_029316858.1 YqzG/YhdC family protein -
  RS399_RS19615 (RS399_19615) tapA 3874288..3875052 (+) 765 WP_084993730.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40484.68 Da        Isoelectric Point: 9.1889

>NTDB_id=888049 RS399_RS19570 WP_003236962.1 3869457..3870527(+) (comGA) [Bacillus inaquosorum strain SI3]
MDSIEKISRTLIEEAYITKASDIHIVPRERDAIIHFRVDHALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLEEGHVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIQQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRSTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=888049 RS399_RS19570 WP_003236962.1 3869457..3870527(+) (comGA) [Bacillus inaquosorum strain SI3]
TTGGATTCAATAGAAAAGATAAGCAGAACCTTGATTGAAGAGGCATATATAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGTGATGCCATCATTCATTTTCGGGTTGATCATGCATTGTTGAAAAAAAGGAACATGAAAAAAGAAGAGTGTG
TAAGACTGATTTCACATTTTAAATTTCTCTCAGCGATGGACATAGGGGAAAGACGAAAGCCGCAAAATGGTTCGCTTACG
TTAAAGCTTGAAGAGGGACATGTTCATTTAAGAATGTCAACGCTCCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATATAATATCCCTTCGATTGATAAACTGTCATTATTTCCGAAGACAGGAGCCACCTTGCTCTCATTTTTAA
AACATTCCCACGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACTACACTATATTCTCTTGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACACTGGAGGACCCTGTTGAAACAAGGGACGAGGATGTTCTTCAGGTACA
GGTGAATGAAAAAGCGGGTGTGACATATTCTACAGGGCTGAAGGCTATTTTGCGCCATGACCCGGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCCAAAATTGCGGTGCGGGCAGCGATGACGGGGCATTTGGTGCTGACGAGCCTTCAC
ACGAGAGATGCGAAGGGCGCGATTTACAGGCTGCTTGAATTCGGCATCAATATGAATGAAATCCAACAGACCGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCGGTGTATTGCCGCCAGTCAC
GAAGCACCAGGAGAGCAAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAGTATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTTGGCTACTTGACGACAAACAATTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.966
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362