Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RS960_RS07475 Genome accession   NZ_CP135966
Coordinates   1538049..1539011 (-) Length   320 a.a.
NCBI ID   WP_317528597.1    Uniprot ID   -
Organism   Staphylococcus aureus strain ST5477     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533049..1544011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS960_RS07435 (RS960_07435) gcvT 1533550..1534641 (-) 1092 WP_262538681.1 glycine cleavage system aminomethyltransferase GcvT -
  RS960_RS07440 (RS960_07440) - 1534800..1535324 (-) 525 WP_001015120.1 shikimate kinase -
  RS960_RS07445 (RS960_07445) - 1535314..1535460 (-) 147 WP_001789879.1 hypothetical protein -
  RS960_RS07450 (RS960_07450) comGF 1535557..1536054 (-) 498 WP_258411170.1 competence type IV pilus minor pilin ComGF Machinery gene
  RS960_RS07455 (RS960_07455) comGE 1535972..1536271 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  RS960_RS07460 (RS960_07460) comGD 1536258..1536704 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  RS960_RS07465 (RS960_07465) comGC 1536682..1536993 (-) 312 WP_262538680.1 competence type IV pilus major pilin ComGC Machinery gene
  RS960_RS07470 (RS960_07470) comGB 1537007..1538077 (-) 1071 WP_262538679.1 competence type IV pilus assembly protein ComGB Machinery gene
  RS960_RS07475 (RS960_07475) comGA 1538049..1539011 (-) 963 WP_317528597.1 competence type IV pilus ATPase ComGA Machinery gene
  RS960_RS07480 (RS960_07480) - 1539063..1539686 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RS960_RS07485 (RS960_07485) - 1539683..1540012 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RS960_RS07490 (RS960_07490) - 1540012..1540998 (-) 987 WP_000161315.1 ROK family glucokinase -
  RS960_RS07495 (RS960_07495) - 1540995..1541198 (-) 204 WP_000087561.1 YqgQ family protein -
  RS960_RS07500 (RS960_07500) - 1541179..1542642 (-) 1464 WP_250743365.1 rhomboid family protein -
  RS960_RS07505 (RS960_07505) - 1542654..1543193 (-) 540 WP_000164547.1 5-formyltetrahydrofolate cyclo-ligase -
  RS960_RS07510 (RS960_07510) rpmG 1543402..1543551 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 320 a.a.        Molecular weight: 36453.51 Da        Isoelectric Point: 9.0368

>NTDB_id=887886 RS960_RS07475 WP_317528597.1 1538049..1539011(-) (comGA) [Staphylococcus aureus strain ST5477]
MKILFQEIINKAIEMKASDVHFIPVKNKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIY
FLKISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISI
EDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHAIDCKGAILRLL
EMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI

Nucleotide


Download         Length: 963 bp        

>NTDB_id=887886 RS960_RS07475 WP_317528597.1 1538049..1539011(-) (comGA) [Staphylococcus aureus strain ST5477]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGA
AGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTCAATAAAATATAT
TTTTTGAAAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCAATTTTTTCAACA
ACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGGCCAA
CTGGTTCAGGAAAGAGTACATTAATGTACCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAATGTAATTTCTATA
GAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAACTATGTAAATTC
GTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTA
TACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAATTGATTGTAAAGGTGCTATTTTAAGGCTATTA
GAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAACTACTATTAAGCA
ACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCAT
CATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATGGATAAATACATT
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

98.148

100

0.994
  comGA Staphylococcus aureus N315

98.148

100

0.994