Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RP314_RS07785 Genome accession   NZ_CP135199
Coordinates   1489559..1490629 (+) Length   356 a.a.
NCBI ID   WP_070082113.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML21-47     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1484559..1495629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP314_RS07755 (RP314_07755) - 1485034..1485279 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  RP314_RS07760 (RP314_07760) - 1485516..1485896 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RP314_RS07770 (RP314_07770) - 1486102..1486932 (+) 831 WP_070082115.1 STAS domain-containing protein -
  RP314_RS07775 (RP314_07775) - 1486970..1488271 (-) 1302 WP_077722596.1 hemolysin family protein -
  RP314_RS07780 (RP314_07780) - 1488417..1489367 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  RP314_RS07785 (RP314_07785) comGA 1489559..1490629 (+) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  RP314_RS07790 (RP314_07790) comGB 1490616..1491653 (+) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  RP314_RS07795 (RP314_07795) comGC 1491700..1491966 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  RP314_RS07800 (RP314_07800) comGD 1491956..1492393 (+) 438 WP_077722595.1 competence type IV pilus minor pilin ComGD Machinery gene
  RP314_RS07805 (RP314_07805) comGE 1492377..1492691 (+) 315 WP_077722594.1 competence type IV pilus minor pilin ComGE Machinery gene
  RP314_RS07810 (RP314_07810) comGF 1492600..1493100 (+) 501 WP_232213409.1 competence type IV pilus minor pilin ComGF -
  RP314_RS07815 (RP314_07815) comGG 1493101..1493478 (+) 378 WP_077722592.1 competence type IV pilus minor pilin ComGG Machinery gene
  RP314_RS07820 (RP314_07820) - 1493535..1493714 (+) 180 WP_003153093.1 YqzE family protein -
  RP314_RS07825 (RP314_07825) - 1493754..1494083 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  RP314_RS07830 (RP314_07830) tapA 1494342..1495013 (+) 672 WP_070082109.1 amyloid fiber anchoring/assembly protein TapA -
  RP314_RS07835 (RP314_07835) sipW 1494985..1495569 (+) 585 WP_012117977.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.91 Da        Isoelectric Point: 9.5862

>NTDB_id=886200 RP314_RS07785 WP_070082113.1 1489559..1490629(+) (comGA) [Bacillus velezensis strain CMML21-47]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=886200 RP314_RS07785 WP_070082113.1 1489559..1490629(+) (comGA) [Bacillus velezensis strain CMML21-47]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCGGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374