Detailed information NTG0886196

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RP314_RS07360
• Genome accession: NZ_CP135199
• Coordinates: 1413507..1414121 (+)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain CMML21-47
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1408507..1419121

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RP314_RS07325 (RP314_07325)	aroE	1409194..1410036 (+)	843	WP_015417858.1	shikimate dehydrogenase	-
RP314_RS07330 (RP314_07330)	yhbY	1410030..1410320 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
RP314_RS07335 (RP314_07335)	-	1410331..1410900 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
RP314_RS07340 (RP314_07340)	yqeK	1410890..1411450 (+)	561	WP_077722612.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RP314_RS07345 (RP314_07345)	rsfS	1411468..1411824 (+)	357	WP_003152864.1	ribosome silencing factor	-
RP314_RS07350 (RP314_07350)	-	1411821..1412558 (+)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
RP314_RS07355 (RP314_07355)	comER	1412627..1413448 (-)	822	WP_014305449.1	late competence protein ComER	-
RP314_RS07360 (RP314_07360)	comEA	1413507..1414121 (+)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
RP314_RS07365 (RP314_07365)	-	1414188..1414757 (+)	570	WP_003152868.1	ComE operon protein 2	-
RP314_RS07370 (RP314_07370)	comEC	1414758..1417109 (+)	2352	WP_077722611.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RP314_RS07375 (RP314_07375)	-	1417128..1417262 (-)	135	WP_003152870.1	YqzM family protein	-
RP314_RS07380 (RP314_07380)	-	1417303..1417457 (+)	155	Protein_1444	hypothetical protein	-
RP314_RS07385 (RP314_07385)	holA	1417497..1418538 (+)	1042	Protein_1445	DNA polymerase III subunit delta	-
RP314_RS07390 (RP314_07390)	rpsT	1418555..1418821 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=886196 RP314_RS07360 WP_013352942.1 1413507..1414121(+) (comEA) [Bacillus velezensis strain CMML21-47]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=886196 RP314_RS07360 WP_013352942.1 1413507..1414121(+) (comEA) [Bacillus velezensis strain CMML21-47]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363