Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RNZ43_RS12250 Genome accession   NZ_CP135143
Coordinates   2505918..2506988 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain L33a     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2500918..2511988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNZ43_RS12200 sipW 2500978..2501562 (-) 585 WP_012117977.1 signal peptidase I SipW -
  RNZ43_RS12205 tapA 2501534..2502205 (-) 672 WP_416405691.1 amyloid fiber anchoring/assembly protein TapA -
  RNZ43_RS12210 - 2502464..2502793 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  RNZ43_RS12215 - 2502833..2503012 (-) 180 WP_003153093.1 YqzE family protein -
  RNZ43_RS12220 comGG 2503069..2503446 (-) 378 WP_416405692.1 competence type IV pilus minor pilin ComGG Machinery gene
  RNZ43_RS12225 comGF 2503447..2503947 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  RNZ43_RS12230 comGE 2503856..2504170 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  RNZ43_RS12235 comGD 2504154..2504591 (-) 438 WP_416405693.1 competence type IV pilus minor pilin ComGD Machinery gene
  RNZ43_RS12240 comGC 2504581..2504889 (-) 309 WP_327969171.1 competence type IV pilus major pilin ComGC Machinery gene
  RNZ43_RS12245 comGB 2504894..2505931 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  RNZ43_RS12250 comGA 2505918..2506988 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  RNZ43_RS12255 - 2507180..2508130 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  RNZ43_RS12260 - 2508276..2509577 (+) 1302 WP_070082114.1 hemolysin family protein -
  RNZ43_RS12265 - 2509615..2510445 (-) 831 WP_070082115.1 STAS domain-containing protein -
  RNZ43_RS12275 - 2510651..2511031 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RNZ43_RS12280 - 2511268..2511513 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=885600 RNZ43_RS12250 WP_003153083.1 2505918..2506988(-) (comGA) [Bacillus velezensis strain L33a]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=885600 RNZ43_RS12250 WP_003153083.1 2505918..2506988(-) (comGA) [Bacillus velezensis strain L33a]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACAGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374