Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RNI18_RS18915 Genome accession   NZ_CP134775
Coordinates   3743057..3744127 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain KB21     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3738057..3749127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS18885 (RNI18_18885) - 3738532..3738777 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  RNI18_RS18890 (RNI18_18890) - 3739013..3739393 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RNI18_RS18900 (RNI18_18900) - 3739599..3740429 (+) 831 WP_003153080.1 STAS domain-containing protein -
  RNI18_RS18905 (RNI18_18905) - 3740468..3741769 (-) 1302 WP_014305416.1 hemolysin family protein -
  RNI18_RS18910 (RNI18_18910) - 3741915..3742865 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  RNI18_RS18915 (RNI18_18915) comGA 3743057..3744127 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  RNI18_RS18920 (RNI18_18920) comGB 3744114..3745151 (+) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  RNI18_RS18925 (RNI18_18925) comGC 3745156..3745464 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  RNI18_RS18930 (RNI18_18930) comGD 3745454..3745891 (+) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  RNI18_RS18935 (RNI18_18935) comGE 3745875..3746189 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  RNI18_RS18940 (RNI18_18940) comGF 3746203..3746598 (+) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  RNI18_RS18945 (RNI18_18945) comGG 3746599..3746976 (+) 378 WP_046559875.1 competence type IV pilus minor pilin ComGG Machinery gene
  RNI18_RS18950 (RNI18_18950) - 3747033..3747212 (+) 180 WP_003153093.1 YqzE family protein -
  RNI18_RS18955 (RNI18_18955) - 3747252..3747581 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  RNI18_RS18960 (RNI18_18960) tapA 3747840..3748511 (+) 672 WP_046559874.1 amyloid fiber anchoring/assembly protein TapA -
  RNI18_RS18965 (RNI18_18965) sipW 3748483..3749067 (+) 585 WP_046559873.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=883616 RNI18_RS18915 WP_003153083.1 3743057..3744127(+) (comGA) [Bacillus velezensis strain KB21]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=883616 RNI18_RS18915 WP_003153083.1 3743057..3744127(+) (comGA) [Bacillus velezensis strain KB21]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374