Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RL452_RS07510 Genome accession   NZ_CP134620
Coordinates   1545771..1546745 (-) Length   324 a.a.
NCBI ID   WP_000697229.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Sa9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1540771..1551745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RL452_RS07470 (RL452_07460) gcvT 1541272..1542363 (-) 1092 WP_000093341.1 glycine cleavage system aminomethyltransferase GcvT -
  RL452_RS07475 (RL452_07465) - 1542522..1543046 (-) 525 WP_001015120.1 shikimate kinase -
  RL452_RS07480 (RL452_07470) - 1543036..1543182 (-) 147 WP_078062659.1 hypothetical protein -
  RL452_RS07485 (RL452_07475) comGF 1543279..1543776 (-) 498 WP_052658450.1 competence type IV pilus minor pilin ComGF Machinery gene
  RL452_RS07490 (RL452_07480) comGE 1543694..1543993 (-) 300 WP_045157654.1 hypothetical protein Machinery gene
  RL452_RS07495 (RL452_07485) comGD 1543980..1544426 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  RL452_RS07500 (RL452_07490) comGC 1544404..1544715 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RL452_RS07505 (RL452_07495) comGB 1544729..1545799 (-) 1071 Protein_1469 competence type IV pilus assembly protein ComGB -
  RL452_RS07510 (RL452_07500) comGA 1545771..1546745 (-) 975 WP_000697229.1 competence type IV pilus ATPase ComGA Machinery gene
  RL452_RS07515 (RL452_07505) - 1546797..1547420 (-) 624 WP_338595134.1 MBL fold metallo-hydrolase -
  RL452_RS07520 (RL452_07510) - 1547417..1547746 (-) 330 WP_001018873.1 MTH1187 family thiamine-binding protein -
  RL452_RS07525 (RL452_07515) - 1547746..1548732 (-) 987 WP_000161315.1 ROK family glucokinase -
  RL452_RS07530 (RL452_07520) - 1548729..1548932 (-) 204 WP_000087561.1 YqgQ family protein -
  RL452_RS07535 (RL452_07525) - 1548913..1550376 (-) 1464 WP_001017855.1 rhomboid family intramembrane serine protease -
  RL452_RS07540 (RL452_07530) - 1550388..1550927 (-) 540 WP_000164549.1 5-formyltetrahydrofolate cyclo-ligase -
  RL452_RS07545 (RL452_07535) rpmG 1551136..1551285 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36873.98 Da        Isoelectric Point: 8.8508

>NTDB_id=882443 RL452_RS07510 WP_000697229.1 1545771..1546745(-) (comGA) [Staphylococcus aureus strain Sa9]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=882443 RL452_RS07510 WP_000697229.1 1545771..1546745(-) (comGA) [Staphylococcus aureus strain Sa9]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAAAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGTATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997