Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RL451_RS02365 Genome accession   NZ_CP134617
Coordinates   437423..437920 (+) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IPLA16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432423..442920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RL451_RS02325 (RL451_02325) - 432467..433453 (+) 987 WP_000161314.1 ROK family glucokinase -
  RL451_RS02330 (RL451_02330) - 433453..433782 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RL451_RS02335 (RL451_02335) - 433779..434402 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RL451_RS02340 (RL451_02340) comGA 434454..435428 (+) 975 WP_331385828.1 competence type IV pilus ATPase ComGA Machinery gene
  RL451_RS02345 (RL451_02345) comGB 435400..436470 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RL451_RS02350 (RL451_02350) comGC 436484..436795 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RL451_RS02355 (RL451_02355) comGD 436773..437219 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RL451_RS02360 (RL451_02360) comGE 437206..437505 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  RL451_RS02365 (RL451_02365) comGF 437423..437920 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  RL451_RS02370 (RL451_02370) - 438017..438163 (+) 147 WP_072399793.1 hypothetical protein -
  RL451_RS02375 (RL451_02375) - 438153..438677 (+) 525 WP_001015120.1 shikimate kinase -
  RL451_RS02380 (RL451_02380) gcvT 438836..439927 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  RL451_RS02385 (RL451_02385) gcvPA 439947..441293 (+) 1347 WP_043854778.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RL451_RS02390 (RL451_02390) gcvPB 441286..442758 (+) 1473 WP_043854779.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=882352 RL451_RS02365 WP_001829055.1 437423..437920(+) (comGF) [Staphylococcus aureus strain IPLA16]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=882352 RL451_RS02365 WP_001829055.1 437423..437920(+) (comGF) [Staphylococcus aureus strain IPLA16]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982