Detailed information NTG0880567

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RHP75_RS10195
• Genome accession: NZ_CP134499
• Coordinates: 2120670..2121896 (+)
• Length: 408 a.a.
• NCBI ID: WP_311091726.1
• Uniprot ID: -
• Organism: Pseudomonas sp. SG20056
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2115670..2126896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHP75_RS10170 (RHP75_10170)	-	2116813..2117121 (-)	309	WP_311091721.1	AzlD domain-containing protein	-
RHP75_RS10175 (RHP75_10175)	-	2117118..2117828 (-)	711	WP_311091722.1	AzlC family ABC transporter permease	-
RHP75_RS10180 (RHP75_10180)	sph	2118213..2119727 (-)	1515	WP_311091723.1	sphingomyelin phosphodiesterase	-
RHP75_RS10185 (RHP75_10185)	-	2119861..2120229 (-)	369	WP_311091724.1	MmcQ/YjbR family DNA-binding protein	-
RHP75_RS10190 (RHP75_10190)	-	2120248..2120526 (-)	279	WP_160014225.1	peptidylprolyl isomerase	-
RHP75_RS10195 (RHP75_10195)	pilU	2120670..2121896 (+)	1227	WP_311091726.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RHP75_RS10200 (RHP75_10200)	-	2121907..2122569 (-)	663	WP_311091727.1	transporter substrate-binding domain-containing protein	-
RHP75_RS10205 (RHP75_10205)	-	2122723..2124678 (-)	1956	WP_311091728.1	acetoacetate--CoA ligase	-
RHP75_RS10210 (RHP75_10210)	-	2124895..2125797 (-)	903	WP_311091729.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 44652.99 Da
• Isoelectric Point: 5.7533

>NTDB_id=880567 RHP75_RS10195 WP_311091726.1 2120670..2121896(+) (pilU) [Pseudomonas sp. SG20056]
MDLNAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSAEPLKAGEVAAIADSVMDSAQREEFERELEMNLAISIASVGR
FRINIFKQRNEVSIVARNIKMDIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTIDGKRRAAVEVLLGTPTVRDLIKRNEFSEIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEEEAVKNADSANNVRLKLKLYRDNPGTSAPAAPAPVAAAPTPAAPAAPAASAGEWGLELKLEDIEEEQLP
EDPGRKGI

Nucleotide

• Length: 1227 bp

>NTDB_id=880567 RHP75_RS10195 WP_311091726.1 2120670..2121896(+) (pilU) [Pseudomonas sp. SG20056]
ATGGACCTCAATGCCATGCTGAAAATCCTGTCCAGCCAGGACGGGTCCGACCTTTATCTTTCCACCGGGGCGCCGCCCTG
CGCCAAGTTTAATGGCGTGCTCAAGGCGCTCAGTGCTGAGCCACTAAAGGCCGGCGAGGTGGCGGCGATTGCCGATTCGG
TGATGGATAGCGCGCAGCGTGAGGAGTTCGAGCGTGAGCTGGAAATGAACCTGGCCATTTCGATTGCCAGCGTTGGGCGC
TTTCGTATCAACATCTTCAAGCAGCGCAACGAAGTGTCGATCGTGGCGCGCAATATCAAGATGGACATTCCTAAGTTCGA
AGACCTCAAGCTGCCGGAAGTGCTGCTCAAGACGGTGATGGAAAAACGCGGTCTGGTGTTGTTTGTCGGTGGCACGGGGT
CGGGCAAGTCGACCTCACTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCTGTGGAGTATGTGCACAAGCACAAGAAGTCGATCATCAACCAGCGCGAAGTGGGCGTGGACACGCGCAGCTTTCACGC
GGCGCTGAAGAACACCCTGCGTCAGGCCCCGGACGTGATTCTGATCGGCGAAATTCGCGACCGCGAGACCATGGAGCATG
CCCTGGCCTTTGCTGACACCGGCCACCTGGCAATCTCCACGTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGTCCGCAGCTGCTCAATGACCTGGGCAACAACCTCAAGGCATTTGTCTCGCAGCG
GCTGGTCAAGACCATCGATGGCAAGCGGCGGGCGGCGGTGGAGGTGTTACTGGGGACGCCAACGGTGCGAGATCTGATCA
AACGCAACGAGTTTTCCGAGATCAAGGAAATCATGGAAAAGTCGAAAAACCTCGGCATGCAGACCTTCGATCAGGCGCTG
ATCGACCTGGTCAACGATGGCTCGATTGATGAAGAGGAAGCGGTGAAAAACGCCGATTCGGCGAACAATGTGCGCCTCAA
GCTCAAGCTCTACCGCGACAACCCCGGTACCTCCGCGCCTGCCGCCCCCGCGCCAGTAGCTGCTGCACCGACGCCTGCTG
CGCCTGCGGCCCCGGCCGCTTCGGCGGGTGAGTGGGGGCTGGAGTTGAAGCTGGAAGATATTGAAGAAGAGCAACTGCCG
GAGGATCCTGGGCGCAAAGGTATTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.742	87.5	0.488
pilU	Vibrio cholerae strain A1552	51.524	88.48	0.456
pilU	Acinetobacter baylyi ADP1	52.841	86.275	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.846	95.343	0.38