Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   RJW53_RS08860 Genome accession   NZ_CP134488
Coordinates   1755248..1756540 (-) Length   430 a.a.
NCBI ID   WP_012774986.1    Uniprot ID   -
Organism   Streptococcus suis strain NLS50     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1750248..1761540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJW53_RS08850 (RJW53_08850) hpf 1753971..1754513 (-) 543 WP_004194117.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RJW53_RS08855 (RJW53_08855) comFC/cflB 1754590..1755255 (-) 666 WP_004194119.1 ComF family protein Machinery gene
  RJW53_RS08860 (RJW53_08860) comFA/cflA 1755248..1756540 (-) 1293 WP_012774986.1 DEAD/DEAH box helicase Machinery gene
  RJW53_RS08865 (RJW53_08865) - 1756597..1757229 (+) 633 WP_014636235.1 YigZ family protein -
  RJW53_RS08870 (RJW53_08870) cysK 1757322..1758248 (+) 927 WP_012774985.1 cysteine synthase A -
  RJW53_RS08875 (RJW53_08875) - 1758274..1758645 (-) 372 WP_002937017.1 S1 RNA-binding domain-containing protein -
  RJW53_RS08880 (RJW53_08880) - 1758647..1760047 (-) 1401 WP_024406021.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  RJW53_RS08885 (RJW53_08885) - 1760097..1760738 (-) 642 WP_002937025.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48975.59 Da        Isoelectric Point: 9.0603

>NTDB_id=880336 RJW53_RS08860 WP_012774986.1 1755248..1756540(-) (comFA/cflA) [Streptococcus suis strain NLS50]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSCFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTVSSLVQIAGRVGRSMERPTG
LLQFYHEGSTGAIEKAVAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=880336 RJW53_RS08860 WP_012774986.1 1755248..1756540(-) (comFA/cflA) [Streptococcus suis strain NLS50]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAATTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTCTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCGAGCAGCAGACAATGCGGTCAAGGAAGCTGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGTAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTTCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGTGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CCAATCATTACCTCTACACAGTGTCAAGTCTTGTTCAGATTGCAGGCCGAGTCGGAAGGAGTATGGAACGTCCGACTGGT
TTACTCCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAGTCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(294-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

65.661

100

0.658
  comFA Lactococcus lactis subsp. cremoris KW2

53.904

92.326

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

37.875

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.349

96.512

0.36