Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJD40_RS01985 Genome accession   NZ_CP134280
Coordinates   401126..402157 (+) Length   343 a.a.
NCBI ID   WP_418113857.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain 21FBVib0106     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396126..407157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD40_RS01950 (RJD40_01955) hemW 396377..397552 (-) 1176 WP_418113853.1 radical SAM family heme chaperone HemW -
  RJD40_RS01955 (RJD40_01960) - 397556..398152 (-) 597 WP_009385807.1 XTP/dITP diphosphatase -
  RJD40_RS01960 (RJD40_01965) - 398165..398596 (-) 432 WP_418113854.1 DUF4426 domain-containing protein -
  RJD40_RS01965 (RJD40_01970) yggU 398627..398917 (-) 291 WP_406732560.1 DUF167 family protein YggU -
  RJD40_RS01970 (RJD40_01975) - 398917..399474 (-) 558 WP_065429768.1 YggT family protein -
  RJD40_RS01975 (RJD40_01980) proC 399516..400334 (-) 819 WP_418113855.1 pyrroline-5-carboxylate reductase -
  RJD40_RS01980 (RJD40_01985) - 400385..401101 (-) 717 WP_418113856.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJD40_RS01985 (RJD40_01990) pilT 401126..402157 (+) 1032 WP_418113857.1 type IV pilus twitching motility protein PilT Machinery gene
  RJD40_RS01990 (RJD40_01995) pilU 402172..403272 (+) 1101 WP_418113858.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJD40_RS01995 (RJD40_02000) - 403390..404721 (+) 1332 WP_005593006.1 IS4 family transposase -
  RJD40_RS02000 (RJD40_02005) lysC 404771..406123 (-) 1353 WP_418113859.1 lysine-sensitive aspartokinase 3 -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37805.30 Da        Isoelectric Point: 6.0872

>NTDB_id=879628 RJD40_RS01985 WP_418113857.1 401126..402157(+) (pilT) [Vibrio scophthalmi strain 21FBVib0106]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPAFSHADVHRLIFEVMNDAQRSEFEEHLEVDFSFELPDIGR
FRVNAFNQARGSAAVFRTIPTSIPTLEQLEAPAIFESIVNLEKGLVLVTGPTGSGKSTTLAAMVDYINQNHNKHILTIED
PIEFVHSNQKCLVNQREVHRDTRSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPGSEKGMVRSMLSESLRSVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSVIQTGSAQGMQTMEQNA
KLLIAKGLVDQAEVEQKIGSLDF

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=879628 RJD40_RS01985 WP_418113857.1 401126..402157(+) (pilT) [Vibrio scophthalmi strain 21FBVib0106]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTTCTCCGAT
GGTGCGCATTGATGGCGAAGTTCGCAAGCTGGGAATCCCTGCTTTTAGCCACGCCGATGTTCATCGCTTGATTTTTGAAG
TGATGAACGATGCTCAGCGTAGCGAATTTGAAGAACACTTAGAAGTTGATTTTTCATTTGAACTTCCCGATATCGGTCGT
TTTCGTGTTAATGCGTTTAATCAAGCGCGCGGCAGTGCAGCGGTTTTTCGTACTATTCCTACCTCAATTCCAACGTTGGA
GCAGCTTGAAGCGCCGGCGATATTTGAAAGTATCGTTAACTTGGAAAAAGGCTTAGTGCTGGTGACGGGCCCTACGGGTT
CTGGTAAGTCGACCACATTGGCAGCGATGGTTGATTATATTAATCAAAACCATAACAAGCATATTCTTACCATTGAAGAT
CCGATTGAATTTGTTCATAGCAATCAAAAATGTTTGGTCAACCAACGTGAAGTACATCGAGATACTCGCAGCTTTAAAGC
GGCTTTGCGTAGTGCTTTACGTGAAGATCCTGATGTCATTCTTGTAGGTGAGTTGCGTGATCAAGAAACGATTAGTTTGG
CGCTAACGGCAGCGGAAACGGGCCACTTAGTTTTTGCAACGCTGCACACCAGCTCTGCAGCCAAAACCATTGACCGTATT
ATTGATGTTTTTCCGGGCAGTGAAAAAGGCATGGTGCGTTCTATGCTTTCGGAATCACTACGTTCAGTGATTGCACAAAA
ACTGCTGAAGCGTATTGGCGGTGGTCGCGTTGCGTGTCATGAAATTATGATGGCGACGCCTGCAATCCGTAACTTGATCC
GTGAAGATAAAGTCGCGCAGATGTATTCAGTGATCCAAACTGGATCGGCTCAAGGCATGCAAACTATGGAACAAAATGCC
AAACTTTTGATCGCTAAGGGCTTGGTCGATCAAGCTGAAGTTGAGCAAAAAATTGGCTCGCTAGATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

86.982

98.542

0.857
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.982

98.542

0.857
  pilT Pseudomonas stutzeri DSM 10701

68.546

98.251

0.673
  pilT Pseudomonas aeruginosa PAK

68.546

98.251

0.673
  pilT Acinetobacter baumannii D1279779

69.605

95.918

0.668
  pilT Acinetobacter baumannii strain A118

69.605

95.918

0.668
  pilT Acinetobacter baylyi ADP1

69.394

96.21

0.668
  pilT Acinetobacter nosocomialis M2

69.301

95.918

0.665
  pilT Legionella pneumophila strain Lp02

67.674

96.501

0.653
  pilT Legionella pneumophila strain ERS1305867

67.674

96.501

0.653
  pilT Neisseria meningitidis 8013

64.242

96.21

0.618
  pilT Neisseria gonorrhoeae MS11

63.939

96.21

0.615
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.887

92.711

0.481
  pilU Vibrio cholerae strain A1552

44.201

93.003

0.411
  pilU Pseudomonas stutzeri DSM 10701

42.073

95.627

0.402
  pilU Acinetobacter baylyi ADP1

40

93.294

0.373