Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RJD40_RS01990 Genome accession   NZ_CP134280
Coordinates   402172..403272 (+) Length   366 a.a.
NCBI ID   WP_418113858.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain 21FBVib0106     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 397172..408272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD40_RS01955 (RJD40_01960) - 397556..398152 (-) 597 WP_009385807.1 XTP/dITP diphosphatase -
  RJD40_RS01960 (RJD40_01965) - 398165..398596 (-) 432 WP_418113854.1 DUF4426 domain-containing protein -
  RJD40_RS01965 (RJD40_01970) yggU 398627..398917 (-) 291 WP_406732560.1 DUF167 family protein YggU -
  RJD40_RS01970 (RJD40_01975) - 398917..399474 (-) 558 WP_065429768.1 YggT family protein -
  RJD40_RS01975 (RJD40_01980) proC 399516..400334 (-) 819 WP_418113855.1 pyrroline-5-carboxylate reductase -
  RJD40_RS01980 (RJD40_01985) - 400385..401101 (-) 717 WP_418113856.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJD40_RS01985 (RJD40_01990) pilT 401126..402157 (+) 1032 WP_418113857.1 type IV pilus twitching motility protein PilT Machinery gene
  RJD40_RS01990 (RJD40_01995) pilU 402172..403272 (+) 1101 WP_418113858.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJD40_RS01995 (RJD40_02000) - 403390..404721 (+) 1332 WP_005593006.1 IS4 family transposase -
  RJD40_RS02000 (RJD40_02005) lysC 404771..406123 (-) 1353 WP_418113859.1 lysine-sensitive aspartokinase 3 -
  RJD40_RS02005 (RJD40_02010) - 406587..407708 (+) 1122 WP_418113860.1 pyridoxal-phosphate-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41021.83 Da        Isoelectric Point: 5.8360

>NTDB_id=879629 RJD40_RS01990 WP_418113858.1 402172..403272(+) (pilU) [Vibrio scophthalmi strain 21FBVib0106]
MDAFLQGMSEQKASDLYITVGAPVLYRVDGELQPQGDKLGEQEVFALLNSLMDSERLSEFKRSREANFAVVRDCGRFRVS
AFFQREQPGAVIRRIETKIPTCEELNLPTVLQELATTKRGLVLVVGATGSGKSTTMAAMTGYRNQHRSGHILTVEDPIEF
VHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMAFAETGHLCMATLHANNANQALERILHLV
PKEKKEQFLFDLSMNLKGVVAQQLLRDKNGQGRHGVFEVLLNSPRIADLIRRGELHEVKATMAKSQQTGMQTFDQSLFQL
LVDGKISEEDALHSADSANDLRIMLKMERGESLTSRSLADITIDMD

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=879629 RJD40_RS01990 WP_418113858.1 402172..403272(+) (pilU) [Vibrio scophthalmi strain 21FBVib0106]
ATGGATGCCTTCCTGCAAGGAATGAGTGAGCAAAAAGCGTCAGATTTGTATATCACCGTTGGCGCTCCAGTGCTTTATCG
TGTTGATGGTGAATTACAGCCACAAGGCGATAAGCTTGGTGAGCAAGAGGTGTTCGCGTTATTGAACTCGTTAATGGATA
GCGAGCGCTTGAGCGAGTTTAAACGCAGTCGTGAAGCGAATTTTGCCGTGGTGCGAGATTGCGGACGATTTCGTGTTTCT
GCCTTTTTTCAACGTGAGCAACCCGGAGCGGTAATTCGTCGTATCGAAACGAAGATCCCAACTTGTGAAGAACTTAATCT
ACCTACGGTGTTGCAAGAATTGGCGACCACCAAACGAGGTTTAGTGCTGGTGGTTGGTGCGACTGGTTCTGGTAAATCAA
CCACCATGGCGGCAATGACTGGCTATCGAAATCAACACCGCAGCGGTCACATTCTCACCGTGGAAGATCCGATTGAATTT
GTTCACGAACATCAGCGCTGCATTGTGACTCAGCGCGAAGTCGGCCTCGATACGGAAAGCTATGAAGTGGCATTGAAGAA
TTCATTACGCCAAGCGCCAGACATGATCTTAATTGGTGAAATTCGCAGTCGAGAAACCATGGAATATGCGATGGCGTTTG
CGGAAACCGGCCATCTGTGTATGGCAACTTTGCATGCCAATAATGCCAATCAAGCGTTAGAGCGTATTTTACATTTGGTG
CCAAAAGAGAAAAAAGAGCAGTTCTTATTTGATTTGTCGATGAACCTGAAAGGCGTGGTTGCTCAGCAGTTGTTGCGAGA
TAAAAATGGTCAAGGTCGACATGGTGTTTTTGAAGTGCTCCTCAATAGCCCTCGTATTGCAGATTTGATCCGTCGTGGTG
AGTTGCACGAAGTGAAAGCGACCATGGCGAAATCGCAGCAGACGGGTATGCAGACTTTTGACCAGTCGTTATTTCAGTTG
CTGGTTGATGGCAAAATTAGTGAAGAGGATGCGCTGCACAGTGCTGATTCAGCCAATGATTTACGCATTATGCTTAAAAT
GGAGCGCGGAGAGTCTCTAACTAGCAGGTCTCTTGCTGATATCACCATTGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.694

100

0.817
  pilU Pseudomonas stutzeri DSM 10701

57.459

98.907

0.568
  pilU Acinetobacter baylyi ADP1

54.467

94.809

0.516
  pilT Legionella pneumophila strain Lp02

42.683

89.617

0.383
  pilT Legionella pneumophila strain ERS1305867

42.683

89.617

0.383
  pilT Acinetobacter baylyi ADP1

42.724

88.251

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.265

90.71

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.641

89.891

0.374
  pilT Pseudomonas aeruginosa PAK

41.337

89.891

0.372
  pilT Acinetobacter baumannii strain A118

41.231

88.798

0.366
  pilT Acinetobacter baumannii D1279779

41.231

88.798

0.366
  pilT Acinetobacter nosocomialis M2

41.231

88.798

0.366
  pilT Vibrio cholerae strain A1552

40.867

88.251

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.867

88.251

0.361