Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RI106_RS02415 Genome accession   NZ_CP134071
Coordinates   450590..451087 (+) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 4233     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445590..456087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI106_RS02375 (RI106_02375) - 445634..446620 (+) 987 WP_000161314.1 ROK family glucokinase -
  RI106_RS02380 (RI106_02380) - 446620..446949 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RI106_RS02385 (RI106_02385) - 446946..447569 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RI106_RS02390 (RI106_02390) comGA 447621..448595 (+) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  RI106_RS02395 (RI106_02395) comGB 448567..449637 (+) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  RI106_RS02400 (RI106_02400) comGC 449651..449962 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RI106_RS02405 (RI106_02405) comGD 449940..450386 (+) 447 WP_310745934.1 competence type IV pilus minor pilin ComGD Machinery gene
  RI106_RS02410 (RI106_02410) comGE 450373..450672 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  RI106_RS02415 (RI106_02415) comGF 450590..451087 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  RI106_RS02420 (RI106_02420) - 451184..451330 (+) 147 Protein_444 hypothetical protein -
  RI106_RS02425 (RI106_02425) - 451320..451844 (+) 525 WP_001015123.1 shikimate kinase -
  RI106_RS02430 (RI106_02430) gcvT 452003..453094 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  RI106_RS02435 (RI106_02435) gcvPA 453114..454460 (+) 1347 WP_043854778.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RI106_RS02440 (RI106_02440) gcvPB 454453..455925 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=878678 RI106_RS02415 WP_001829055.1 450590..451087(+) (comGF) [Staphylococcus aureus strain 4233]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=878678 RI106_RS02415 WP_001829055.1 450590..451087(+) (comGF) [Staphylococcus aureus strain 4233]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982