Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RIF20_RS05910 Genome accession   NZ_CP134068
Coordinates   1154579..1155076 (+) Length   165 a.a.
NCBI ID   WP_029642894.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 63     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1149579..1160076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIF20_RS05880 (RIF20_05880) - 1149939..1151585 (+) 1647 WP_000277718.1 IS1182 family transposase -
  RIF20_RS05885 (RIF20_05885) comGA 1151610..1152584 (+) 975 Protein_1122 competence type IV pilus ATPase ComGA -
  RIF20_RS05890 (RIF20_05890) comGB 1152556..1153626 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RIF20_RS05895 (RIF20_05895) comGC 1153640..1153951 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RIF20_RS05900 (RIF20_05900) comGD 1153929..1154375 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RIF20_RS05905 (RIF20_05905) comGE 1154362..1154661 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  RIF20_RS05910 (RIF20_05910) comGF 1154579..1155076 (+) 498 WP_029642894.1 competence type IV pilus minor pilin ComGF Machinery gene
  RIF20_RS05915 (RIF20_05915) - 1155173..1155319 (+) 147 WP_001789879.1 hypothetical protein -
  RIF20_RS05920 (RIF20_05920) - 1155309..1155833 (+) 525 WP_001015123.1 shikimate kinase -
  RIF20_RS05925 (RIF20_05925) gcvT 1155992..1157083 (+) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  RIF20_RS05930 (RIF20_05930) gcvPA 1157103..1158449 (+) 1347 WP_000019689.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RIF20_RS05935 (RIF20_05935) gcvPB 1158442..1159914 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19196.92 Da        Isoelectric Point: 10.2192

>NTDB_id=878618 RIF20_RS05910 WP_029642894.1 1154579..1155076(+) (comGF) [Staphylococcus aureus strain 63]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=878618 RIF20_RS05910 WP_029642894.1 1154579..1155076(+) (comGF) [Staphylococcus aureus strain 63]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(70-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976